#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA4684500	Binding	Inhibition of recombinant human N-terminal GST-tagged DOT1L (1 to 420 residues) expressed in Escherichia coli BL21 (DE3) using histone as substrate incubated for 1 hr by MTase-glo based luminescence assay	Homo sapiens	15	ALA4680176	assay format	Scientific Literature
2.	ALA4684501	Binding	Binding affinity to recombinant human N-terminal GST-tagged DOT1L (1 to 420 residues) expressed in Escherichia coli BL21 (DE3) assessed as increase in melting temperature incubated for 1 min by 1,8-ANS probe based thermal shift assay	Homo sapiens	1	ALA4680176	assay format	Scientific Literature
3.	ALA4684502	Functional	Antiproliferative activity against mouse bone marrow cell transformed with MLL-AF9 assessed as reduction in cell growth measured upto 11 days by hemocytometry	Mus musculus	1	ALA4680176	cell-based format	Scientific Literature
4.	ALA4684503	Functional	Antiproliferative activity against mouse bone marrow cell transformed with MLL-AF9 assessed as reduction in cell growth measured after 6 days by hemocytometry	Mus musculus	1	ALA4680176	cell-based format	Scientific Literature
5.	ALA4684504	Functional	Antiproliferative activity against mouse bone marrow cell transformed with MLL-AF9 assessed as reduction in cell growth measured after 9 days by hemocytometry	Mus musculus	1	ALA4680176	cell-based format	Scientific Literature
6.	ALA4684505	Functional	Antiproliferative activity against mouse bone marrow cell transformed with MLL-AF9 assessed as reduction in cell growth measured after 11 days by hemocytometry	Mus musculus	1	ALA4680176	cell-based format	Scientific Literature
7.	ALA4684506	Functional	Antiproliferative activity against mouse bone marrow cell transformed with MLL-AF9 assessed as reduction in cell growth at 30 uM measured after 11 days by hemocytometry relative to control	Mus musculus	1	ALA4680176	cell-based format	Scientific Literature
8.	ALA4684507	Functional	Antiproliferative activity against mouse bone marrow cell transformed with E2A-HLF assessed as reduction in cell growth at 1 to 30 uM measured after 15 days by hemocytometry	Mus musculus	1	ALA4680176	cell-based format	Scientific Literature
9.	ALA4684508	Binding	Inhibition of DOT1L in mouse bone marrow cell transformed with MLL-AF9 cells assessed as reduction in H3K79 methylation measured after 9 days by Western blot analysis	Mus musculus	1	ALA4680176	tissue-based format	Scientific Literature
10.	ALA4684509	Binding	Inhibition of DOT1L in mouse bone marrow cell transformed with MLL-AF9 cells assessed as reduction in H3K79 methylation at 30 uM measured after 9 days by Western blot analysis relative to control	Mus musculus	1	ALA4680176	tissue-based format	Scientific Literature
11.	ALA4684510	Binding	Inhibition of DOT1L in mouse bone marrow cell transformed with MLL-AF9 cells assessed as reduction in H3K79 methylation measured after 6 days by Western blot analysis	Mus musculus	1	ALA4680176	tissue-based format	Scientific Literature
12.	ALA4684511	Binding	Inhibition of DOT1L in mouse bone marrow cell transformed with MLL-AF9 cells assessed as reduction in H3K4me3 methylation at 1 to 30 uM measured after 6 days by Western blot analysis	Mus musculus	1	ALA4680176	tissue-based format	Scientific Literature
13.	ALA4684512	Binding	Inhibition of DOT1L in mouse bone marrow cell transformed with MLL-AF9 cells assessed as reduction in H3K20me2 methylation at 1 to 30 uM measured after 6 days by Western blot analysis	Mus musculus	1	ALA4680176	tissue-based format	Scientific Literature
14.	ALA4684513	Functional	Induction of cell differentiation in mouse bone marrow cells transformed with MLL-AF9 assessed as change in morphology incubated for 6 days by Wright-Giemsa staining based inverted microscopy	Mus musculus	1	ALA4680176	cell-based format	Scientific Literature
15.	ALA4684515	Binding	Inhibition of recombinant human DNMT1 using poly(dl-dC) as substrate by hotspot assay	Homo sapiens	7	ALA4680176	single protein format	Scientific Literature
16.	ALA4684516	Binding	Inhibition of recombinant human EZH2 using core histone as substrate by hotspot assay	Homo sapiens	7	ALA4680176	single protein format	Scientific Literature
17.	ALA4684517	Binding	Inhibition of recombinant human G9a using histone H3 (1 to 21) as substrate by hotspot assay	Homo sapiens	7	ALA4680176	single protein format	Scientific Literature
18.	ALA4684518	Binding	Inhibition of recombinant human SET7/9 using core histone as substrate by hotspot assay	Homo sapiens	7	ALA4680176	single protein format	Scientific Literature
19.	ALA4684519	Binding	Inhibition of recombinant human PRMT3 using histone H4 as substrate by hotspot assay	Homo sapiens	7	ALA4680176	single protein format	Scientific Literature
20.	ALA4684520	Binding	Inhibition of recombinant human PRMT5 using histone H2A as substrate by hotspot assay	Homo sapiens	7	ALA4680176	single protein format	Scientific Literature