# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4676462 | Functional | Anticoagulant activity in human platelet-rich plasma assessed as reduction in TF-induced thrombin generation by measuring endogenous thrombin potential at 100 uM preincubated for 15 mins followed by TF addition by Z-Gly-Gly-Arg-AMC substrate based calibrated automated thrombography (Rvb = 1203 +/- 44 AU.min/L) | Homo sapiens | 3 | ALA4673255 | cell-free format | Scientific Literature | |
2. | ALA4676463 | Functional | Anticoagulant activity in human platelet-rich plasma assessed as reduction in TF-induced thrombin generation by measuring Tmax of clotting time at 100 uM preincubated for 15 mins followed by TF addition by Z-Gly-Gly-Arg-AMC substrate based calibrated automated thrombography (Rvb = 8.1 +/- 0.6 min) | Homo sapiens | 3 | ALA4673255 | cell-free format | Scientific Literature | |
3. | ALA4676464 | Functional | Anticoagulant activity in human platelet-rich plasma assessed as reduction in TF-induced thrombin generation by measuring half life of clotting time at 100 uM preincubated for 15 mins followed by TF addition by Z-Gly-Gly-Arg-AMC substrate based calibrated automated thrombography (Rvb= 3 +/- 0.5 min) | Homo sapiens | 3 | ALA4673255 | cell-free format | Scientific Literature | |
4. | ALA4676465 | Functional | Anticoagulant activity in human platelet-rich plasma assessed as reduction in TF-induced thrombin generation by measuring amplitude of thrombin peak at 100 uM preincubated for 15 mins followed by TF addition by Z-Gly-Gly-Arg-AMC substrate based calibrated automated thrombography (Rvb = 63 +/- 3.7 AU/L) | Homo sapiens | 3 | ALA4673255 | cell-free format | Scientific Literature | |
5. | ALA4676476 | Functional | Antiplatelet activity in human platelet-rich plasma assessed as inhibition of MDA-MB-231-induced platelet aggregation at 0.1 to 10 uM by light transmission aggregometry | Homo sapiens | 2 | ALA4673255 | cell-free format | Scientific Literature | |
6. | ALA4676478 | Binding | Time dependent inhibition of human coagulation factor XIIA using Boc-Gln-Gly-Arg-AMC as fluorogenic substrate at 0.5 to 132 uM by fluorescence assay | Homo sapiens | 1 | ALA4673255 | single protein format | Scientific Literature | |
7. | ALA4676479 | Functional | Anticoagulant activity in human blood assessed as concentration required to double the activated partial thromboplastin time incubated for 1 min followed by aPTT reagent based assay | Homo sapiens | 2 | ALA4673255 | tissue-based format | Scientific Literature | |
8. | ALA4676480 | Binding | Selectivity index, ratio of IC50 for inhibition of human coagulation factor alpha-thrombin using Boc-Val-Pro-Arg-AMC as fluorogenic substrate to IC50 for inhibition of human coagulation factor XIIA using Boc-Gln-Gly-Arg-AMC as fluorogenic substrate incubated for 1 hr by fluorometric assay | Homo sapiens | 5 | ALA4673255 | assay format | Scientific Literature | |
9. | ALA4676486 | Binding | Inhibition of human coagulation factor XIA using Mes-dArg-Pro-Arg-AMC as fluorogenic substrate measured at 1 min interval for 1 hr by fluorometric assay | Homo sapiens | 5 | ALA4673255 | single protein format | Scientific Literature | |
10. | ALA4676487 | ADME | Cytotoxicity against human HepG2 cells assessed as reduction in cell viability at 100 uM incubated for 48 hrs by alamar blue assay relative to control | Homo sapiens | 3 | ALA4673255 | cell-based format | Scientific Literature | |
11. | ALA4676489 | Binding | Inhibition of human coagulation factor alpha thrombin in presence of Boc-Val-Pro-Arg-AMC as fluorogenic substrate at 1 uM measured at 1 min interval for 1 hr by fluorometric assay relative to control | Homo sapiens | 25 | ALA4673255 | single protein format | Scientific Literature | |
12. | ALA4676490 | Binding | Inhibition of human coagulation factor XIIA in presence of Boc-Gln-Gly-Arg-AMC as fluorogenic substrate at 1 uM measured at 1 min interval for 1 hr by fluorometric assay relative to control | Homo sapiens | 25 | ALA4673255 | single protein format | Scientific Literature | |
13. | ALA4676491 | Binding | Inhibition of human uPA using Tos-Gly-Pro-Arg-AMC as fluorogenic substrate measured at 1 min interval for 1 hr by fluorometric assay | Homo sapiens | 4 | ALA4673255 | single protein format | Scientific Literature | |
14. | ALA4676493 | Binding | Inhibition of human plasmin using Mes-dArg-Phe-Arg-AMC as fluorogenic substrate measured at 1 min interval for 1 hr by fluorometric assay | Homo sapiens | 5 | ALA4673255 | single protein format | Scientific Literature | |
15. | ALA4676494 | Binding | Inhibition of human kallikrein using Mes-dArg-Pro-Arg-AMC as fluorogenic substrate measured at 1 min interval for 1 hr by fluorometric assay | Homo sapiens | 5 | ALA4673255 | single protein format | Scientific Literature | |
16. | ALA4676495 | Binding | Inhibition of human recombinant MAGL using 1,3-dihydroxypropan-2-yl 4-pyren-1-ylbutanoate as substrate measured after 45 mins by reverse-phase HPLC-based fluorescence assay | Homo sapiens | 4 | ALA4673255 | single protein format | Scientific Literature | |
17. | ALA4676496 | Binding | Inhibition of FAAH in rat brain membrane using N-(2-hydroxyethyl)-4-pyren-1-ylbutanamide as substrate measured after 60 mins by reverse-phase HPLC-based fluorescence assay | Rattus norvegicus | 4 | ALA4673255 | cell membrane format | Scientific Literature | |
18. | ALA4676497 | Binding | Inhibition of human coagulation factor thrombin using Boc-Val-Pro-Arg-AMC as fluorogenic substrate at 100 nM measured at 1 min interval for 1 hr by fluorometric assay relative to control | Homo sapiens | 4 | ALA4673255 | single protein format | Scientific Literature | |
19. | ALA4676498 | Binding | Inhibition of human coagulation factor XIIA using Boc-Gln-Gly-Arg-AMC as fluorogenic substrate at 100 nM measured at 1 min interval for 1 hr by fluorometric assay relative to control | Homo sapiens | 4 | ALA4673255 | single protein format | Scientific Literature | |
20. | ALA4676500 | Functional | Anticoagulant activity in human blood assessed as increase in activated partial thromboplastin time at 300 uM incubated for 1 min by aPTT reagent based assay relative to control | Homo sapiens | 25 | ALA4673255 | tissue-based format | Scientific Literature |