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# Aladdin ID Assay Type Description Organism Compounds Reference BAO Format Source 1. ALA4731475 ADME Prodrug activation in DMSO assessed as H2O2 mediated prodrug release after 4 hrs in presence of 0.1 uM H2O2 after 4 hrs by RP-HPLC-UV analysis 1 ALA4725469 assay format Scientific Literature 2. ALA4731476 ADME Prodrug activation in DMSO/PBS assessed as H2O2 mediated prodrug release after 4 hrs in presence of 100 to 500 uM H2O2 by RP-HPLC-UV analysis 1 ALA4725469 assay format Scientific Literature 3. ALA4731477 ADME Prodrug activation in DMSO/PBS assessed as H2O2 mediated prodrug release after 4 hrs in presence of => 1000 uM H2O2 by RP-HPLC-UV analysis 1 ALA4725469 assay format Scientific Literature 4. ALA4731478 ADME Prodrug activation in DMSO/PBS assessed as H2O2 mediated prodrug release after 0.5 hrs in presence of H2O2 by RP-HPLC-UV analysis 1 ALA4725469 assay format Scientific Literature 5. ALA4731479 ADME Prodrug activation in DMSO/PBS assessed as H2O2 mediated prodrug release by measuring (Z)-2-((5-(1-cyclohexyl-1,6-dihydroimidazo[4,5-d]pyrrolo[2,3-b]pyridin-2-yl)furan-2-yl)(hydroxy)methyl)-3-(dimethylamino)-3-((4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)benzyl)oxy)acrylonitrile level after 0.5 hrs in presence of H2O2 by RP-HPLC-UV analysis 1 ALA4725469 assay format Scientific Literature 6. ALA4731480 ADME Prodrug activation in DMSO/PBS assessed as H2O2 mediated prodrug release after 3.5 hrs in presence of H2O2 by RP-HPLC-UV analysis 1 ALA4725469 assay format Scientific Literature 7. ALA4731481 ADME Stability in mouse plasma at 20 uM after 0.25 to 24 hrs by RP-UPLC-UV analysis Mus musculus 1 ALA4725469 cell-free format Scientific Literature 8. ALA4731482 ADME Stability in human plasma at 20 uM after 0.25 to 24 hrs by RP-UPLC-UV analysis Homo sapiens 1 ALA4725469 cell-free format Scientific Literature 9. ALA4731483 Unassigned Stability in simulated intestinal fluid incubated for 0.25 to 24 hrs by RP-UPLC-UV analysis 1 ALA4725469 assay format Scientific Literature 10. ALA4731484 Unassigned Stability in simulated gastric fluid measured after 0.25 to 24 hrs by RP-UPLC-UV analysis 1 ALA4725469 assay format Scientific Literature 11. ALA4731485 Physicochemical Stability in phosphate buffer saline at pH 7.4 after 0.25 to 24 hrs by RP-UPLC-UV analysis 1 ALA4725469 small-molecule physicochemical format Scientific Literature 12. ALA4731486 ADME Inhibition of JAK3 in mouse BaF3 cells assessed as reduction in cell viability by MTT assay Mus musculus 3 ALA4725469 cell-based format Scientific Literature 13. ALA4731487 ADME Inhibition of JAK3 in mouse BaF3 cells assessed as reduction in cell viability in presence of 0.1 mM H2O2 by MTT assay Mus musculus 2 ALA4725469 cell-based format Scientific Literature 14. ALA4731488 Physicochemical Dissociation constant, pKa of the compound 2 ALA4725469 small-molecule physicochemical format Scientific Literature 15. ALA4731489 Physicochemical Aqueous solubility of compound at pH 7.4 2 ALA4725469 small-molecule physicochemical format Scientific Literature 16. ALA4731490 ADME Permeability of compound at pH 7.4 by PAMPA assay 2 ALA4725469 assay format Scientific Literature 17. ALA4731491 Binding Inhibition of JAK3 in mouse BaF3/TEL-JAK3 cells assessed as effect on STAT3 phosphorylation at 0.125 to 2 uM by Western blot analysis Mus musculus 2 ALA4725469 cell-based format Scientific Literature 18. ALA4731492 Binding Inhibition of JAK3 in mouse BaF3/TEL-JAK3 cells assessed as effect on STAT5 phosphorylation at 0.125 to 2 uM by Western blot analysis Mus musculus 2 ALA4725469 cell-based format Scientific Literature 19. ALA4731493 ADME Half life in Sprague-Dawley rat at 20 mg/kg, ip measured for 0.8 to 24 hrs by LC-MS/MS analysis Rattus norvegicus 2 ALA4725469 organism-based format Scientific Literature 20. ALA4731494 ADME Cmax in Sprague-Dawley rat at 20 mg/kg, ip measured for 0.8 to 24 hrs by LC-MS/MS analysis Rattus norvegicus 2 ALA4725469 organism-based format Scientific Literature