# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4729810 | Binding | Inhibition of recombinant human wild-type N-terminal His-tagged PDIA1 expressed in Escherichia coli strain BL21(DE3) using bovine insulin as substrate preincubated for 1 hr followed by substrate addition | Homo sapiens | 108 | ALA4725388 | assay format | Scientific Literature | |
2. | ALA4729814 | Functional | Cytotoxicity against human U-87 MG cells assessed as growth inhibition measured after 72 hrs by MTT assay | Homo sapiens | 106 | ALA4725388 | cell-based format | Scientific Literature | |
3. | ALA4729818 | Binding | Binding affinity to recombinant human wild-type N-terminal His-tagged PDIA1 expressed in Escherichia coli strain BL21(DE3) assessed as increase in protein mass at 100 uM measured after 30 mins in presence of GSTO1 and GRP78 by Q-TOF MS analysis relative to control | Homo sapiens | 2 | ALA4725388 | assay format | Scientific Literature | |
4. | ALA4729820 | Binding | Displacement of PACMA57 from recombinant human wild-type N-terminal His-tagged PDIA1 expressed in Escherichia coli strain BL21(DE3) at 100 uM measured after overnight incubation by gel-based imaging analysis | Homo sapiens | 2 | ALA4725388 | assay format | Scientific Literature |