# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4712494 | Binding | Inhibition of oligonucleotide [32P]-labelled 5'-AGCITCATTTCCCGTAAATCCCTA probe binding to STAT3 SH2 domain in mouse NIH3T3/v-Src nuclear extract preincubated for 30 mins followed by hSIE probe addition by EMSA analysis | Mus musculus | 37 | ALA4706639 | subcellular format | Scientific Literature | |
2. | ALA4712495 | Functional | Antiproliferative activity against human MDA-MB-231 cells assessed as reduction in cell viability incubated for 72 hrs by CyQuant assay | Homo sapiens | 47 | ALA4706639 | cell-based format | Scientific Literature | |
3. | ALA4712496 | Functional | Antiproliferative activity against human MDA-MB-468 cells harbouring STAT3 assessed as reduction in cell viability incubated for 72 hrs by CyQuant assay | Homo sapiens | 42 | ALA4706639 | cell-based format | Scientific Literature | |
4. | ALA4712497 | Functional | Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability incubated for 72 hrs by CyQuant assay | Homo sapiens | 44 | ALA4706639 | cell-based format | Scientific Literature | |
5. | ALA4712498 | ADME | Cytotoxicity against human MCF-10A cells assessed as reduction in cell viability incubated for 72 hrs by CyQuant assay | Homo sapiens | 42 | ALA4706639 | cell-based format | Scientific Literature | |
6. | ALA4712504 | Binding | Binding affinity to STAT3 (unknown origin) assessed as dissociation constant by isothermal titration calorimetry | Homo sapiens | 2 | ALA4706639 | single protein format | Scientific Literature | |
7. | ALA4712505 | Binding | Binding affinity to STAT3 (unknown origin) assessed as change in gibbs free energy by isothermal titration calorimetry | Homo sapiens | 2 | ALA4706639 | single protein format | Scientific Literature | |
8. | ALA4712506 | Binding | Binding affinity to STAT3 (unknown origin) assessed as change in enthalpy by isothermal titration calorimetry | Homo sapiens | 2 | ALA4706639 | single protein format | Scientific Literature | |
9. | ALA4712507 | Binding | Binding affinity to STAT3 (unknown origin) assessed as change in entropy by isothermal titration calorimetry | Homo sapiens | 2 | ALA4706639 | single protein format | Scientific Literature | |
10. | ALA4712513 | Binding | Inhibition of oligonucleotide [32P]-labelled 5'-AGCITCATTTCCCGTAAATCCCTA hSIE probe binding to STAT3 in human MDA-MB-468 cell nuclear extract at 5 uM by EMSA analysis | Homo sapiens | 3 | ALA4706639 | tissue-based format | Scientific Literature | |
11. | ALA4712514 | Binding | Inhibition of STAT3 phosphorylation at Y705 residues in human MDA-MB-231 whole cell lysate by immunoblotting analysis | Homo sapiens | 1 | ALA4706639 | subcellular format | Scientific Literature | |
12. | ALA4712518 | Binding | Inhibition of STAT3 in human MDA-MB-231 cells assessed as effect on EGFR expression at 1 to 3 uM incubated for 3 to 24 hrs by immunoblotting analysis | Homo sapiens | 1 | ALA4706639 | cell-based format | Scientific Literature | |
13. | ALA4712519 | Binding | Inhibition of STAT3 in human MDA-MB-231 cells assessed as effect on JAK2 expression at 1 to 3 uM incubated for 3 to 24 hrs by immunoblotting analysis | Homo sapiens | 1 | ALA4706639 | cell-based format | Scientific Literature | |
14. | ALA4712520 | Binding | Inhibition of STAT3 in human MDA-MB-231 cells assessed as effect on Src expression at 1 to 3 uM incubated for 3 to 24 hrs by immunoblotting analysis | Homo sapiens | 1 | ALA4706639 | cell-based format | Scientific Literature | |
15. | ALA4712521 | Binding | Inhibition of STAT3 in human MDA-MB-231 cells assessed as effect on Akt expression at 1 to 3 uM incubated for 3 to 24 hrs by immunoblotting analysis | Homo sapiens | 1 | ALA4706639 | cell-based format | Scientific Literature | |
16. | ALA4712522 | Binding | Inhibition of STAT3 in human MDA-MB-231 cells assessed as effect on Erk1/2 expression at 1 to 3 uM incubated for 3 to 24 hrs by immunoblotting analysis | Homo sapiens | 1 | ALA4706639 | cell-based format | Scientific Literature | |
17. | ALA4712523 | Functional | Anticlonogenic activity against human MDA-MB-231 cells assessed as inhibition of colony formation at 0.5 uM incubated for 4 hrs by crystal violet staining based assay | Homo sapiens | 1 | ALA4706639 | cell-based format | Scientific Literature | |
18. | ALA4712526 | Binding | Inhibition of STAT3 in human MDA-MB-231 cells assessed as inhibition of c-myc expression at 1 to 3 uM incubated for 3 to 24 hrs by immunoblotting analysis | Homo sapiens | 1 | ALA4706639 | cell-based format | Scientific Literature | |
19. | ALA4712527 | Binding | Inhibition of STAT3 in human MDA-MB-231 cells assessed as inhibition of VEGF expression at 1 to 3 uM incubated for 3 to 24 hrs by immunoblotting analysis | Homo sapiens | 1 | ALA4706639 | cell-based format | Scientific Literature | |
20. | ALA4712528 | Binding | Inhibition of STAT3 in human MDA-MB-231 cells assessed as inhibition of Bcl-2 expression at 1 to 3 uM incubated for 3 to 24 hrs by immunoblotting analysis | Homo sapiens | 1 | ALA4706639 | cell-based format | Scientific Literature |