# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4680947 | B | Binding affinity to Mycobacterium tuberculosis CysM by ITC analysis | 4 | assay format | Scientific Literature | |||
2. | ALA4680949 | B | Inhibition of Mycobacterium tuberculosis CysM expressed in Escherichia coli strain BL21 (DE3) assessed as apparent first-order rate constant for the formation of the alpha-aminoacrylate intermediate at 100 uM incubated for 5 mins in presence of OPS by spectrophotometry | Mycobacterium tuberculosis | 19 | assay format | Scientific Literature | ||
3. | ALA4680950 | B | Inhibition of Mycobacterium tuberculosis CysM expressed in Escherichia coli strain BL21 (DE3) assessed as reduction in amino-acrylate intermediate formation by measuring residual enzyme activity at 100 uM incubated for 5 mins in presence of OPS by spectrophotometry | Mycobacterium tuberculosis | 10 | assay format | Scientific Literature | ||
4. | ALA4680951 | F | Antimycobacterial activity against Mycobacterium tuberculosis H37Rv assessed as inhibition of microbial growth incubated for 5 days by alamar blue assay | Mycobacterium tuberculosis | 10 | organism-based format | Scientific Literature | ||
5. | ALA4680952 | F | Antimycobacterial activity against Mycobacterium tuberculosis H37Rv grown under nutrient starvation condition assessed as reduction in nonreplicating cells count incubated for 7 days at 10 ug/ml | Mycobacterium tuberculosis | 7 | organism-based format | Scientific Literature | ||
6. | ALA4680957 | A | Cytotoxicity in human SH-SY5Y cells assessed as reduction in cell viability at 10 uM incubated for 72 hrs by Cell-titer-Glo assay | Homo sapiens | 6 | cell-based format | Scientific Literature | ||
7. | ALA4680958 | A | Cytotoxicity in human HepG2 cells assessed as reduction in cell viability at 10 uM incubated for 72 hrs by Cell-titer-Glo assay | Homo sapiens | 6 | cell-based format | Scientific Literature | ||
8. | ALA4680959 | A | Cytotoxicity in mouse J774.A1 cells assessed as reduction in cell viability at 10 uM incubated for 72 hrs by Cell-titer-Glo assay | Mus musculus | 6 | cell-based format | Scientific Literature | ||
9. | ALA4680960 | A | Cytotoxicity in human THP-1 cells assessed as reduction in cell viability at 10 uM incubated for 72 hrs by Cell-titer-Glo assay | Homo sapiens | 6 | cell-based format | Scientific Literature | ||
10. | ALA5262988 | B | Inhibition of hexahistidine-tagged recombinant Mycobacterium tuberculosis CysK2 expressed in Escherichia coli BL21(DE3) assessed as amount of S-sulfocysteine produced using O-acetyl-L-serine as substrate incubated for 30 mins by acid-ninhydrin based spectrophotometric assay | Mycobacterium tuberculosis | 12 | assay format | Scientific Literature | ||
11. | ALA5262989 | B | Inhibition of hexahistidine-tagged recombinant Mycobacterium tuberculosis CysK1 expressed in Escherichia coli BL21(DE3) assessed as amount of cysteine produced using O-acetyl-L-serine as substrate incubated for 12 mins by acid-ninhydrin based spectrophotometric assay | Mycobacterium tuberculosis | 12 | assay format | Scientific Literature | ||
12. | ALA5262990 | B | Inhibition of hexahistidine-tagged recombinant Mycobacterium tuberculosis CysK2 expressed in Escherichia coli BL21(DE3) assessed as dissociation constant using PLP as substrate incubated for 5 mins by fluorescence analysis | Mycobacterium tuberculosis | 7 | assay format | Scientific Literature |