# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4739181 | Binding | Potency index, ratio of EW-7197 IC50 to test compound IC50 for recombinant human GST-fused ALK5 expressed in baculovirus infected Sf9 cells assessed as incoporation of [33]Pi after 60 mins in presence of [gamma-33P]-ATP by microplate scintillation counter method | Homo sapiens | 2 | ALA4732251 | cell-based format | Scientific Literature | |
2. | ALA4739182 | Binding | Inhibition of recombinant human p38alpha expressed in Escherichia coli assessed as incoporation of [33]Pi after 60 mins in presence of [gamma-33P]-ATP by microplate scintillation counter method | Homo sapiens | 16 | ALA4732251 | assay format | Scientific Literature | |
3. | ALA4739183 | Binding | Inhibition of recombinant human GST-fused ALK5 expressed in baculovirus infected Sf9 cells assessed as incoporation of [33]Pi after 60 mins in presence of [gamma-33P]-ATP by microplate scintillation counter method | Homo sapiens | 16 | ALA4732251 | cell-based format | Scientific Literature | |
4. | ALA4739184 | Functional | Inhibition of epithelial-mesenchymal transition in human U-87MG cells assessed as reduction in TGF-beta stimulated Slug protein expression after 48 hrs by Western blot anlaysis | Homo sapiens | 2 | ALA4732251 | cell-based format | Scientific Literature | |
5. | ALA4739185 | Functional | Inhibition of epithelial-mesenchymal transition in human U-87MG cells assessed as reduction in TGF-beta stimulated nuclear translocation of slug at 1 uM pretreated with compound followed by TGFbeta stimulation and measured after 48 hrs by immunofluorescence assay | Homo sapiens | 1 | ALA4732251 | cell-based format | Scientific Literature | |
6. | ALA4739186 | Functional | Inhibition of TGF-beta-induced morphological changes in human U-87MG cells at 0.1 to 1 uM after 48 hrs | Homo sapiens | 2 | ALA4732251 | cell-based format | Scientific Literature | |
7. | ALA4739187 | Functional | Reversal of TGF-beta-induced epithelial-mesenchymal transition in human U-87MG cells assessed as downregulation of N-cadherin expression at 1 uM after 48 hrs by immunofluorescence assay | Homo sapiens | 1 | ALA4732251 | cell-based format | Scientific Literature | |
8. | ALA4739188 | Functional | Reversal of TGF-beta-induced epithelial-mesenchymal transition in human U-87MG cells assessed as reduction in N-cadherin protein level at 0.1 to 0.5 uM after 48 hrs by Western blotting analysis | Homo sapiens | 2 | ALA4732251 | cell-based format | Scientific Literature | |
9. | ALA4739189 | Functional | Reversal of TGF-beta-induced epithelial-mesenchymal transition in human U-87MG cells assessed as reduction in vimentin protein level at 0.1 to 0.5 uM after 48 hrs by Western blotting analysis | Homo sapiens | 2 | ALA4732251 | cell-based format | Scientific Literature | |
10. | ALA4739190 | Functional | Reversal of TGF-beta-induced epithelial-mesenchymal transition in human U-87MG cells assessed as reduction in N-cadherin mRNA level at 1 uM after 48 hrs by RT-PCR analysis | Homo sapiens | 1 | ALA4732251 | cell-based format | Scientific Literature | |
11. | ALA4739191 | Functional | Reversal of TGF-beta-induced epithelial-mesenchymal transition in human U-87MG cells assessed as reduction in vimentin mRNA level at 1 uM after 48 hrs by RT-PCR analysis | Homo sapiens | 1 | ALA4732251 | cell-based format | Scientific Literature | |
12. | ALA4739192 | Functional | Inhibition of TGF-beta-induced cell migration in human U-87MG cells at 1 uM incubated for 36 hrs by bright field microscopy based wound healing assay | Homo sapiens | 1 | ALA4732251 | cell-based format | Scientific Literature | |
13. | ALA4739193 | Functional | Inhibition of TGF-beta-induced cell invasion in human U-87MG cells at 1 uM incubated for 24 hrs by crystal violet staining based matrigel transwell assay | Homo sapiens | 1 | ALA4732251 | cell-based format | Scientific Literature | |
14. | ALA4739194 | Binding | Potency index, ratio of LY-2157299 IC50 to test compound IC50 for recombinant human GST-fused ALK5 expressed in baculovirus infected Sf9 cells assessed as incoporation of [33]Pi after 60 mins in presence of [gamma-33P]-ATP by microplate scintillation counter method | Homo sapiens | 14 | ALA4732251 | cell-based format | Scientific Literature | |
15. | ALA4739195 | Binding | Potency index, ratio of LY-2157299 IC50 to test compound IC50 for recombinant human p38alpha expressed in Escherichia coli assessed as incoporation of [33]Pi after 60 mins in presence of [gamma-33P]-ATP by microplate scintillation counter method | Homo sapiens | 1 | ALA4732251 | assay format | Scientific Literature | |
16. | ALA4739196 | Binding | Potency index, ratio of EW-7197 IC50 to test compound IC50 for recombinant human p38alpha expressed in Escherichia coli assessed as incoporation of [33]Pi after 60 mins in presence of [gamma-33P]-ATP by microplate scintillation counter method | Homo sapiens | 1 | ALA4732251 | assay format | Scientific Literature |