Binding affinity to recombinant LIMK1 (330 to 637 residues) (unknown origin) expressed in baculovirus infected insect cells assessed as change in melting temperature at 10 uM by differential scanning fluorimetry
Binding affinity to recombinant LIMK2 (330 to 632 residues) (unknown origin) expressed in baculovirus infected insect cells assessed as change in melting temperature at 10 uM by differential scanning fluorimetry
Inhibition of recombinant full length LIMK1 (330 to 637 residues) (unknown origin) expressed in HEK293T cells incubated for 2 hrs in presence of tracer K10 by NanoBRET assay
Inhibition of recombinant full length LIMK2 (330 to 632 residues) (unknown origin) expressed in HEK293T cells incubated for 2 hrs in presence of tracer K10 by NanoBRET assay
Inhibition of recombinant LIMK1 (330 to 637 residues) (unknown origin) expressed in baculovirus infected insect cells using CFL1 as substrate preincubated for 10 mins followed by substrate addition and measured after 60 mins in presence of ATP by RapidFire mass spectrometry
Inhibition of recombinant LIMK2 (330 to 632 residues) (unknown origin) expressed in baculovirus infected insect cells using CFL1 as substrate preincubated for 10 mins followed by substrate addition and measured after 60 mins in presence of ATP by RapidFire mass spectrometry
Inhibition of neurite outgrowth in BMP7 stimulated mouse N1E-115 cells transfected with siFMR1 preincubated for 12 hrs followed by BMP7 stimulation for 24 hrs