| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA4702212 | B | Inhibition of C-terminal His-tagged recombinant human MIF expressed in Escherichia coli BL21 (DE3) using 4-HPP as substrate preincubated for 10 mins followed by substrate addition by UV absorption spectrum analysis | Homo sapiens | 22 | assay format | Scientific Literature | ||
| 2. | ALA4702214 | B | Binding affinity to C-terminal His-tagged recombinant human MIF expressed in Escherichia coli BL21 (DE3) at 200 nM by fluorescence assay | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 3. | ALA4702215 | B | Binding affinity to C-terminal His-tagged recombinant human MIF expressed in Escherichia coli BL21 (DE3) assessed as fluorescence quenching at 50 nM by fluorescence assay | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 4. | ALA4702216 | A | Binding affinity to bovine serum albumin assessed as fluorescence quenching at 50 nM by fluorescence assay | Bos taurus | 1 | single protein format | Scientific Literature | ||
| 5. | ALA4702217 | B | Competitive inhibition of C-terminal His-tagged recombinant human MIF expressed in Escherichia coli BL21 (DE3) up to 50 nM using varying levels of 4-HPP as substrate preincubated for 10 mins followed by substrate addition by Lineweaver-burk plot analysis | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 6. | ALA4702218 | P | Half life of compound in pH 7.4 PBS buffer at 100 nM | 1 | small-molecule physicochemical format | Scientific Literature | |||
| 7. | ALA4702219 | P | Half life of compound in pH 7.4 PBS buffer at 100 nM in presence of MIF | 1 | small-molecule physicochemical format | Scientific Literature | |||
| 8. | ALA4702220 | B | Binding affinity to C-terminal His-tagged recombinant human MIF expressed in Escherichia coli BL21 (DE3) assessed as fluorescence quenching at 50 nM in presence of CuSO4 by fluorescence assay | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 9. | ALA4702222 | B | Binding affinity to C-terminal His-tagged recombinant human MIF expressed in Escherichia coli BL21 (DE3) assessed as inhibition of MIF interaction with MBP-fused human CD74 preincubated for 10 mins with CD74 followed by MIF addition and measured after 30 mins by ELISA | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 10. | ALA4702224 | T | Toxicity in human A549 cells assessed as reduction in cell viability up to 20 uM measured after 24 hrs by MTS assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 11. | ALA4702227 | F | Inhibition of colony formation in human A549 cells at 2.5 to 10 uM incubated for 10 days by crystal violet staining based assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 12. | ALA4702228 | B | Inhibition of MIF (unknown origin) cultured in human A549 cells assessed as reduction in MIF-induced ERk phosphorylation at 10 uM preincubated with MIF for 10 mins followed by cell addition and measured after 10 mins by Western blot analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 13. | ALA4702229 | P | Solubility of compound in pH 7.4 phosphate buffer | 3 | small-molecule physicochemical format | Scientific Literature |
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