| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA4773310 | B | Inhibition of recombinant human 10His-tagged eIF4E expressed in Escherichia coli BL21 (DE3) cells by catalytic enzyme-linked click chemistry assay | Homo sapiens | 3 | cell-based format | Scientific Literature | ||
| 2. | ALA4773311 | B | Binding affinity to recombinant human 10His-tagged eIF4E expressed in Escherichia coli BL21 (DE3) cells assessed as association constant by surface plasmon resonance analysis | Homo sapiens | 3 | cell-based format | Scientific Literature | ||
| 3. | ALA4773312 | B | Binding affinity to recombinant human 10His-tagged eIF4E expressed in Escherichia coli BL21 (DE3) cells assessed as dissociation constant by surface plasmon resonance analysis | Homo sapiens | 3 | cell-based format | Scientific Literature | ||
| 4. | ALA4773313 | B | Binding affinity to recombinant human 10His-tagged eIF4E expressed in Escherichia coli BL21 (DE3) cells assessed as binding constant by surface plasmon resonance analysis | Homo sapiens | 3 | cell-based format | Scientific Literature | ||
| 5. | ALA4773314 | B | Binding affinity to eIF4E in HEK293 cell lysates assessed as thermal stabilization of protein at 1 to 15 uM incubated for 1 hrs followed by heating at 49 to 52.4 degC for 3 mins by CETSA-based Western blot analysis | Homo sapiens | 2 | subcellular format | Scientific Literature | ||
| 6. | ALA4773315 | A | Drug uptake in cytosol of human HeLa cells stably expressing Halo-tagged-GFP assessed as normalized fluorescence in cytosol incubated for 4 hrs by flow cytometry based CAPA | Homo sapiens | 2 | subcellular format | Scientific Literature | ||
| 7. | ALA4773316 | A | Drug uptake in cytosol of human HeLa cells stably expressing Halo-tagged-GFP assessed as normalized fluorescence in cytosol at 0.74 uM incubated for 4 hrs by flow cytometry based CAPA relative to control | Homo sapiens | 2 | subcellular format | Scientific Literature | ||
| 8. | ALA4773317 | B | Inhibition of eIF4E/4E-BP1 interaction in human MDA-MB-231 cells incubated for 6 hr by m7GDP cap pull down assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 9. | ALA4773318 | B | Inhibition of eIF4E/eIF4G interaction in human MDA-MB-231 cells incubated for 6 hr by m7GDP cap pull down assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 10. | ALA4773319 | B | Inhibition of eIF4E/4E-BP1 interaction in human MCF7 cells incubated for 6 hr by m7GDP cap pull down assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 11. | ALA4773320 | B | Inhibition of eIF4E/eIF4G interaction in human MCF7 cells incubated for 6 hr by m7GDP cap pull down assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 12. | ALA4773321 | B | Inhibition of eIF4E/4E-BP1 interaction in human T47D cells incubated for 6 hr by m7GDP cap pull down assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 13. | ALA4773322 | B | Inhibition of eIF4E/eIF4G interaction in human T47D cells incubated for 6 hr by m7GDP cap pull down assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 14. | ALA4773323 | F | Anti-proliferative activity against human MDA-MB-231 cells after 6 days by CellTiter-Glo assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 15. | ALA4773324 | F | Anti-proliferative activity against human MCF7 cells after 6 days by CellTiter-Glo assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 16. | ALA4773325 | F | Anti-proliferative activity against human T47D cells after 6 days by CellTiter-Glo assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 17. | ALA4773326 | A | Stability in mouse plasma assessed as half life at 1 uM incubated for 30 mins by LC-MS/MS analysis | Mus musculus | 1 | cell-free format | Scientific Literature | ||
| 18. | ALA4773327 | B | Inhibition of eIF4E in human MCF7 cells measured up to 42 hrs by m7GDP cap pull down assay | Homo sapiens | 1 | cell-based format | Scientific Literature |
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