# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4685921 | B | Substrate activity at human N-terminal His-tagged SIRT7 (68 to 354 residues) expressed in Escherichia coli at 0.5 uM measured after 2 hrs in presence of NAD+ and trypsin by fluorescence assay | Homo sapiens | 16 | assay format | Scientific Literature | ||
2. | ALA4685922 | B | Substrate activity at human N-terminal His-tagged SIRT6 (1 to 355 residues) expressed in Escherichia coli at 0.5 uM measured after 2 hrs in presence of NAD+ and trypsin by fluorescence assay | Homo sapiens | 16 | assay format | Scientific Literature | ||
3. | ALA4685923 | B | Substrate activity at human N-terminal His-tagged SIRT5 (34 to 302 residues) expressed in Escherichia coli at 0.5 uM measured after 2 hrs in presence of NAD+ and trypsin by fluorescence assay | Homo sapiens | 16 | assay format | Scientific Literature | ||
4. | ALA4685924 | B | Substrate activity at human N-terminal His-tagged SIRT3 (122 to 391 residues) expressed in Escherichia coli at 0.5 uM measured after 2 hrs in presence of NAD+ and trypsin by fluorescence assay | Homo sapiens | 16 | assay format | Scientific Literature | ||
5. | ALA4685925 | B | Substrate activity at human N-terminal His-tagged SIRT2 (56 to 356 residues) expressed in Escherichia coli at 0.5 uM measured after 2 hrs in presence of NAD+ and trypsin by fluorescence assay | Homo sapiens | 16 | assay format | Scientific Literature | ||
6. | ALA4685926 | B | Substrate activity at human N-terminal His-tagged SIRT1 (183 to 664 residues) expressed in Escherichia coli at 0.5 uM measured after 2 hrs in presence of NAD+ and trypsin by fluorescence assay | Homo sapiens | 16 | assay format | Scientific Literature | ||
7. | ALA4685927 | B | Substrate activity at human N-terminal His-tagged SIRT7 (68 to 354 residues) expressed in Escherichia coli at 5 uM measured after 2 hrs in presence of NAD+ and trypsin by fluorescence assay | Homo sapiens | 16 | assay format | Scientific Literature | ||
8. | ALA4685928 | B | Substrate activity at human N-terminal His-tagged SIRT6 (1 to 355 residues) expressed in Escherichia coli at 5 uM measured after 2 hrs in presence of NAD+ and trypsin by fluorescence assay | Homo sapiens | 16 | assay format | Scientific Literature | ||
9. | ALA4685929 | B | Substrate activity at human N-terminal His-tagged SIRT5 (34 to 302 residues) expressed in Escherichia coli at 5 uM measured after 2 hrs in presence of NAD+ and trypsin by fluorescence assay | Homo sapiens | 16 | assay format | Scientific Literature | ||
10. | ALA4685930 | B | Substrate activity at human N-terminal His-tagged SIRT3 (122 to 391 residues) expressed in Escherichia coli at 5 uM measured after 2 hrs in presence of NAD+ and trypsin by fluorescence assay | Homo sapiens | 16 | assay format | Scientific Literature | ||
11. | ALA4685931 | B | Substrate activity at human N-terminal His-tagged SIRT2 (56 to 356 residues) expressed in Escherichia coli at 5 uM measured after 2 hrs in presence of NAD+ and trypsin by fluorescence assay | Homo sapiens | 16 | assay format | Scientific Literature | ||
12. | ALA4685932 | B | Substrate activity at human N-terminal His-tagged SIRT1 (183 to 664 residues) expressed in Escherichia coli at 5 uM measured after 2 hrs in presence of NAD+ and trypsin by fluorescence assay | Homo sapiens | 16 | assay format | Scientific Literature | ||
13. | ALA4685933 | B | Substrate activity at human N-terminal His-tagged SIRT7 (68 to 354 residues) expressed in Escherichia coli at 50 uM measured after 2 hrs in presence of NAD+ and trypsin by fluorescence assay | Homo sapiens | 16 | assay format | Scientific Literature | ||
14. | ALA4685934 | B | Substrate activity at human N-terminal His-tagged SIRT6 (1 to 355 residues) expressed in Escherichia coli at 50 uM measured after 2 hrs in presence of NAD+ and trypsin by fluorescence assay | Homo sapiens | 16 | assay format | Scientific Literature | ||
15. | ALA4685935 | B | Substrate activity at human N-terminal His-tagged SIRT5 (34 to 302 residues) expressed in Escherichia coli at 50 uM measured after 2 hrs in presence of NAD+ and trypsin by fluorescence assay | Homo sapiens | 16 | assay format | Scientific Literature | ||
16. | ALA4685936 | B | Substrate activity at human N-terminal His-tagged SIRT3 (122 to 391 residues) expressed in Escherichia coli at 50 uM measured after 2 hrs in presence of NAD+ and trypsin by fluorescence assay | Homo sapiens | 16 | assay format | Scientific Literature | ||
17. | ALA4685937 | B | Substrate activity at human N-terminal His-tagged SIRT2 (56 to 356 residues) expressed in Escherichia coli at 50 uM measured after 2 hrs in presence of NAD+ and trypsin by fluorescence assay | Homo sapiens | 16 | assay format | Scientific Literature | ||
18. | ALA4685938 | B | Substrate activity at human N-terminal His-tagged SIRT1 (183 to 664 residues) expressed in Escherichia coli at 50 uM measured after 2 hrs in presence of NAD+ and trypsin by fluorescence assay | Homo sapiens | 16 | assay format | Scientific Literature | ||
19. | ALA4685947 | B | Substrate activity at human N-terminal His-tagged SIRT5 (34 to 302 residues) expressed in Escherichia coli assessed as Km measured after 1 hr in presence of NAD+ and trypsin by Michaelis-Menten kinetic model based fluorescence assay | Homo sapiens | 4 | assay format | Scientific Literature | ||
20. | ALA4685948 | B | Substrate activity at human N-terminal His-tagged SIRT5 (34 to 302 residues) expressed in Escherichia coli assessed as Vmax measured after 1 hr in presence of NAD+ and trypsin by Michaelis-Menten kinetic model based fluorescence assay | Homo sapiens | 4 | assay format | Scientific Literature |