# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4771751 | Binding | Inhibition of recombinant human His6/SUMO-tagged HDAC8 expressed in Escherichia coli (BL21) DE3 pLysS assessed as residual activity at 50 uM using Boc-Lys(trifluoroacetyl)-AMC as substrate preincubated for 60 mins followed by substrate addition and measured after 60 mins by fluorescence assay relative to control | Homo sapiens | 24 | ALA4765368 | assay format | Scientific Literature | |
2. | ALA4771753 | Binding | Inhibition of recombinant human N-terminal GST-tagged HDAC4 (627 to end residues) expressed in baculovirus infected Sf9 insect cells assessed as residual activity at 50 uM using Boc-Lys(trifluoroacetyl)-AMC as substrate preincubated for 60 mins followed by substrate addition and measured after 60 mins by fluorescence assay relative to control | Homo sapiens | 24 | ALA4765368 | cell-based format | Scientific Literature |