# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4701743 | Binding | Inhibition of recombinant human full-length N-terminal His-tagged p110 alpha/p85 alpha expressed in baculovirus expression system using PIP2 as substrate preincubated for 15 mins followed by substrate addition and measured after 60 mins by ADP HiLyte 647 tracer bound ADP2 antibody-Tb conjugate based HTRF assay | Homo sapiens | 13 | ALA4699485 | assay format | Scientific Literature | |
2. | ALA4701744 | Functional | Antiproliferative activity against human A2780 cells after 48 hrs by MTT assay | Homo sapiens | 13 | ALA4699485 | cell-based format | Scientific Literature | |
3. | ALA4701746 | Binding | Inhibition of recombinant human GST-tagged mTOR catalytic domain (1360 to 2549 residues) expressed in baculovirus expression system using GFP-4E-BP1 as substrate preincubated for 15 mins followed by substrate addition and measured after 60 mins by TR-FRET assay | Homo sapiens | 5 | ALA4699485 | assay format | Scientific Literature | |
4. | ALA4701747 | Functional | Antiproliferative activity against human PC-3 cells after 48 hrs by MTT assay | Homo sapiens | 5 | ALA4699485 | cell-based format | Scientific Literature | |
5. | ALA4701748 | Functional | Antiproliferative activity against human SK-OV-3 cells after 48 hrs by MTT assay | Homo sapiens | 5 | ALA4699485 | cell-based format | Scientific Literature | |
6. | ALA4701749 | ADME | Metabolic stability in dog liver microsomes assessed as parent compound remaining at 10 uM remaining after 120 mins in presence of NADPH by HPLC analysis | Canis lupus familiaris | 4 | ALA4699485 | microsome format | Scientific Literature | |
7. | ALA4701750 | ADME | Metabolic stability in mouse liver microsomes assessed as parent compound remaining at 10 uM remaining after 120 mins in presence of NADPH by HPLC analysis | Mus musculus | 4 | ALA4699485 | microsome format | Scientific Literature | |
8. | ALA4701751 | ADME | Metabolic stability in human liver microsomes assessed as parent compound remaining at 10 uM remaining after 120 mins in presence of NADPH by HPLC analysis | Homo sapiens | 4 | ALA4699485 | microsome format | Scientific Literature | |
9. | ALA4701752 | ADME | AUC in Swiss mouse at 3 mg/kg, po via gavage by LC-MS/MS analysis | Mus musculus | 4 | ALA4699485 | organism-based format | Scientific Literature | |
10. | ALA4701753 | ADME | Cmax in Swiss mouse at 3 mg/kg, po via gavage by LC-MS/MS analysis | Mus musculus | 4 | ALA4699485 | organism-based format | Scientific Literature | |
11. | ALA4701754 | ADME | Tmax in Swiss mouse at 3 mg/kg, po via gavage by LC-MS/MS analysis | Mus musculus | 4 | ALA4699485 | organism-based format | Scientific Literature | |
12. | ALA4701755 | ADME | Elimination half life in Swiss mouse at 3 mg/kg, po via gavage by LC-MS/MS analysis | Mus musculus | 4 | ALA4699485 | organism-based format | Scientific Literature | |
13. | ALA4701756 | Binding | Channel blockade of human ERG G601S mutant expressed in mammalian expression system assessed as effect on surface expression of channel at 1 to 30 uM by HERG-Lite assay | Homo sapiens | 3 | ALA4699485 | assay format | Scientific Literature | |
14. | ALA4701757 | Binding | Inhibition of human ERG channel trafficking expressed in mammalian expression system at 1 to 30 uM by HERG-Lite assay | Homo sapiens | 3 | ALA4699485 | assay format | Scientific Literature | |
15. | ALA4701758 | Binding | Inhibition of human ERG by patch-clamp assay | Homo sapiens | 3 | ALA4699485 | single protein format | Scientific Literature | |
16. | ALA4701759 | ADME | Inhibition of CYP3A4 in human liver microsomes using isoform-specific probe substrates preincubated for 5 mins followed by NADPH addition by LC-MS/MS analysis | Homo sapiens | 3 | ALA4699485 | microsome format | Scientific Literature | |
17. | ALA4701760 | ADME | Inhibition of CYP2C19 in human liver microsomes using isoform-specific probe substrates preincubated for 5 mins followed by NADPH addition by LC-MS/MS analysis | Homo sapiens | 3 | ALA4699485 | microsome format | Scientific Literature | |
18. | ALA4701761 | ADME | Inhibition of CYP2D6 in human liver microsomes using isoform-specific probe substrates preincubated for 5 mins followed by NADPH addition by LC-MS/MS analysis | Homo sapiens | 3 | ALA4699485 | microsome format | Scientific Literature | |
19. | ALA4701762 | Binding | Inhibition of human AKT1 at 1 uM using fluorescently labeled peptide substrate measured after 3 hrs by microfluidic mobility shift assay relative to control | Homo sapiens | 1 | ALA4699485 | single protein format | Scientific Literature | |
20. | ALA4701763 | Binding | Inhibition of human AKT2 at 1 uM using fluorescently labeled peptide substrate measured after 3 hrs by microfluidic mobility shift assay relative to control | Homo sapiens | 1 | ALA4699485 | single protein format | Scientific Literature |