Inhibition of Wistar rat lens ALR2 assessed as reduction in NADPH oxidation using D,L-glyceraldehyde and NADPH as substrate preincubated for 10 mins followed by substrate addition and measured for 4 mins by spectrophotometric analysis
Inhibition of Wistar rat kidney ALR1 assessed as reduction in NADPH oxidation using sodium D-glucuronate and NADPH as substrate preincubated for 10 mins followed by substrate addition and measured for 4 mins by spectrophotometric analysis
Potency index, ratio of IC50 of epalrestat to IC50 of compound for inhibition of Wistar rat lens ALR2 using D,L-glyceraldehyde and NADPH as substrate preincubated for 10 mins followed by substrate addition and measured for 4 mins by spectrophotometric analysis
Noncompetitive inhibition of Wistar rat lens ALR2 at 0.025 mM using varying level of D,L-glyeraldehyde as substrate in presence of NADPH by double reciprocal plot analysis
Uncompetitive inhibition of Wistar rat lens ALR2 at 2.5 mM using D,L-glyeraldehyde as substrate in presence of varying levels of NADPH by double reciprocal plot analysis
Inhibition of polyol pathway in STZ-induced Kunming mouse model of diabetes assessed as decrease in sorbitol level in kidney cortex at 80 mg/kg, po administered daily via gavage for 8 weeks and measured at 6 hrs post-final drug dose by fluorospectrophotometric analysis
Inhibition of polyol pathway in STZ-induced Kunming mouse model of diabetes assessed as decrease in sorbitol level in kidney cortex at 160 mg/kg, po administered daily via gavage for 8 weeks and measured at 6 hrs post-final drug dose by fluorospectrophotometric analysis
Inhibition of polyol pathway in STZ-induced Kunming mouse model of diabetes assessed as decrease in sorbitol level in kidney cortex at 80 mg/kg, po administered QD via gavage for 4 weeks and measured at 6 hrs post-final drug dose by fluorospectrophotometric analysis
Antioxidant activity in STZ-induced diabetic Kunming mouse assessed as reduction in liver MDA level at 80 mg/kg, po administered daily via gavage for 8 weeks and measured at 6 hrs post-final drug dose relative to STZ-treated group
Antioxidant activity in STZ-induced diabetic Kunming mouse assessed as reduction in liver MDA level at 160 mg/kg, po administered daily via gavage for 8 weeks and measured at 6 hrs post-final drug dose relative to STZ-treated group
Antioxidant activity in STZ-induced diabetic Kunming mouse assessed as reduction in liver MDA level at 80 mg/kg, po administered QD via gavage for 4 weeks and measured at 6 hrs post-final drug dose relative to STZ-treated group
Antioxidant activity in STZ-induced diabetic Kunming mouse assessed as reduction in liver MDA level at 80 mg/kg, po administered daily via gavage for 8 weeks and measured at 6 hrs post-final drug dose relative to normal group
Antioxidant activity in STZ-induced diabetic Kunming mouse assessed as rise in liver MDA level at 160 mg/kg, po administered daily via gavage for 8 weeks and measured at 6 hrs post-final drug dose relative to normal group
Antioxidant activity in STZ-induced diabetic Kunming mouse assessed as rise in liver MDA level at 80 mg/kg, po administered QD via gavage for 4 weeks and measured at 6 hrs post-final drug dose relative to normal group
Antioxidant activity in STZ-induced diabetic Kunming mouse assessed as increase in SOD level in kidney at 80 mg/kg, po administered daily via gavage for 8 weeks and measured at 6 hrs post-final drug dose
Antioxidant activity in STZ-induced diabetic Kunming mouse assessed as increase in SOD level in kidney at 160 mg/kg, po administered daily via gavage for 8 weeks and measured at 6 hrs post-final drug dose
Antioxidant activity in STZ-induced diabetic Kunming mouse assessed as increase in GSH level in kidney at 80 mg/kg, po administered daily via gavage for 8 weeks and measured at 6 hrs post-final drug dose by microplate reader analysis