| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA4731560 | B | Inhibition of N-terminal GST-fused recombinant human EHMT2 (786 to 1210 residues) expressed in Escherichia coli using histone H3 (1 to 21) as substrate at 10 uM by radiometric HotSpot assay relative to control | Homo sapiens | 1 | ALA4725473 | assay format | Scientific Literature | |
| 2. | ALA4731561 | B | Inhibition of full-length human N-terminal FLAG-tagged PRMT5/full length N-terminal His6-tagged MEP50 (unknown origin) expressed in baculovirus infected Sf9 insect cells assessed as reduction of S-adenosyl-L-homocysteine formation using human recombinant histone H2A (1 to 130 residues) and S-adenosyl-L-methionine as substrate after 60 mins by rapidfire mass spectrometry analysis | Homo sapiens | 17 | ALA4725473 | cell-based format | Scientific Literature | |
| 3. | ALA4731562 | B | Inhibition of PRMT5 in human A549 cells assessed as reduction in arginine dimethylation of SmD1/3 after 48 hrs by Hoechst Stain/ CellMask Deep Red Stain based immunohistochemistry method | Homo sapiens | 8 | ALA4725473 | cell-based format | Scientific Literature | |
| 4. | ALA4731563 | B | Binding affinity to N-terminal FLAG-tagged human PRMT5 (1 to 637 residues)/N-terminal thrombin His-tagged MEP50 (2 to 342 residues) expressed in baculovirus infected Sf21 cells by streptavidin coated sensor chip based surface plasmon resonance analysis | Homo sapiens | 1 | ALA4725473 | cell-based format | Scientific Literature | |
| 5. | ALA4731564 | B | Inhibition of recombinant human N-terminal His-tagged MLL1 (3745 to 3969 residues)/human WDR5 (22 to 334)/human RbBP5 (1 to 538)/human Ash2L (2 to 534)/human DPY30 (1 to 99) expressed in Escherichia coli using nucleosome as substrate at 10 uM by radiometric HotSpot assay relative to control | Homo sapiens | 1 | ALA4725473 | assay format | Scientific Literature | |
| 6. | ALA4731565 | B | Inhibition of recombinant human N-terminal GST-tagged PRDM9 (2 to 414 residues) expressed in baculovirus infected Sf9 cells using histone H3 as substrate at 10 uM by radiometric HotSpot assay relative to control | Homo sapiens | 1 | ALA4725473 | cell-based format | Scientific Literature | |
| 7. | ALA4731566 | B | Inhibition of recombinant human N-terminal His-tagged SMYD2 (2 to 433 residues) expressed in Escherichia coli using histone H4 as substrate at 10 uM by radiometric HotSpot assay relative to control | Homo sapiens | 1 | ALA4725473 | assay format | Scientific Literature | |
| 8. | ALA4731567 | B | Inhibition of recombinant human N-terminal GST-tagged SUV420H1 (2 to 393 residues ) expressed in baculovirus infected Sf9 cells using core histone as substrate at 10 uM by radiometric HotSpot assay relative to control | Homo sapiens | 1 | ALA4725473 | cell-based format | Scientific Literature | |
| 9. | ALA4731568 | B | Inhibition of recombinant human full length C-terminal His-tagged SETD7 expressed in Escherichia coli using core histone as substrate at 10 uM by radiometric HotSpot assay relative to control | Homo sapiens | 1 | ALA4725473 | assay format | Scientific Literature | |
| 10. | ALA4731569 | B | Inhibition of recombinant human GST-tagged SETD8 (195 to 352 residues) expressed in Escherichia coli using nucleosome as substrate at 10 uM by radiometric HotSpot assay relative to control | Homo sapiens | 1 | ALA4725473 | assay format | Scientific Literature | |
| 11. | ALA4731570 | B | Inhibition of recombinant human N-terminal His-tagged EZH2 (2 to 746)/human recombinant AEBP2 (2 to 517)/human recombinant EED (2 to 441)/human recombinant RbAp48 (2 to 425)/human recombinant SUZ12 (2 to 739) expressed in baculovirus infected insect cells using core histone as substrate at 10 uM by radiometric HotSpot assay relative to control | Homo sapiens | 1 | ALA4725473 | cell-based format | Scientific Literature | |
| 12. | ALA4731571 | B | Inhibition of recombinant human N- terminal GST-tagged SET1B (1629 to 1923 residues) expressed in Escherichia coli using core histone as substrate at 10 uM by radiometric HotSpot assay relative to control | Homo sapiens | 1 | ALA4725473 | assay format | Scientific Literature | |
| 13. | ALA4731572 | B | Inhibition of recombinant human N- terminal His-tagged MLL2 (5319 to 5537 residues) expressed in Escherichia coli using core histone as substrate at 10 uM by radiometric HotSpot assay relative to control | Homo sapiens | 1 | ALA4725473 | assay format | Scientific Literature | |
| 14. | ALA4731573 | B | Inhibition of recombinant human N-terminal fusion protein tagged and C-terminal His-tagged SUV39H1 (44 to 412 residues) expressed in Escherichia coli using nucleosome as substrate at 10 uM by radiometric HotSpot assay relative to control | Homo sapiens | 1 | ALA4725473 | assay format | Scientific Literature | |
| 15. | ALA4731574 | B | Inhibition of recombinant human C-terminal His-tagged SUV39H2 (48 to 410 residues) expressed in Escherichia coli using nucleosome as substrate at 10 uM by radiometric HotSpot assay relative to control | Homo sapiens | 1 | ALA4725473 | assay format | Scientific Literature | |
| 16. | ALA4731575 | B | Competitive inhibition of full-length human N-terminal FLAG-tagged PRMT5/full length N-terminal His6-tagged MEP50 (unknown origin) expressed in baculovirus infected Sf9 insect cells assessed as reduction of S-adenosyl-L-homocysteine formation using varying concentrations of human recombinant histone H2A (1 to 130 residues) and S-adenosyl-L-methionine as substrate after 80 mins by rapidfire mass spectrometry analysis | Homo sapiens | 2 | ALA4725473 | cell-based format | Scientific Literature | |
| 17. | ALA4731576 | B | Non-competitive inhibition of full-length human N-terminal FLAG-tagged PRMT5/full length N-terminal His6-tagged MEP50 (unknown origin) expressed in baculovirus infected Sf9 insect cells assessed as reduction of S-adenosyl-L-homocysteine formation using human recombinant histone H2A (1 to 130 residues) and varying concentrations of S-adenosyl-L-methionine as substrate after 80 mins by rapidfire mass spectrometry analysis | Homo sapiens | 2 | ALA4725473 | cell-based format | Scientific Literature | |
| 18. | ALA4731577 | B | Inhibition of recombinant human N-terminal GST-tagged DOT1L (1 to 416 residues) expressed in Escherichia coli using nucleosome as substrate at 10 uM by radiometric HotSpot assay relative to control | Homo sapiens | 1 | ALA4725473 | assay format | Scientific Literature | |
| 19. | ALA4731578 | B | Inhibition of recombinat human N-terminal Flag-tagged PRMT5 (2 to 637 residues)/ N-terminal His-tagged MEP50 (2 to 342 residues) (unknown origin) expressed in baculovirus infected Sf9 cells using recombinant human histone H2A (1 to 130 residues) as substrate at 10 uM by radiometric HotSpot assay relative to control | Homo sapiens | 1 | ALA4725473 | cell-based format | Scientific Literature | |
| 20. | ALA4731579 | B | Inhibition of recombinant human N-terminal His-tagged full length PRMT7 (2 to 692 residues) expressed in baculovirus system using GST-GAR as substrate at 10 uM by radiometric HotSpot assay relative to control | Homo sapiens | 1 | ALA4725473 | assay format | Scientific Literature |
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