# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4771188 | B | Inhibition of N7-MTase activity of N-terminal His6-tagged SARS-CoV nsp14 expressed in Escherichia coli using GpppAC4 and [3H]SAM as substrate at 50 uM preincubated with enzyme followed by substrate addition and measured after 30 mins by filter binding assay | Severe acute respiratory syndrome-related coronavirus | 17 | assay format | Scientific Literature | ||
2. | ALA4771189 | B | Inhibition of N7-MTase activity of Vaccinia virus mRNA capping enzyme D1/D12 complex using GpppAC4 and [3H]SAM as substrate at 50 uM preincubated with enzyme followed by substrate addition and measured after 30 mins by filter binding assay | Vaccinia virus | 17 | assay format | Scientific Literature | ||
3. | ALA4771190 | A | Inhibition of N-terminal His6-tagged human RNA-N7 MTase expressed in Escherichia coli using GpppAC4 and [3H]SAM as substrate at 50 uM preincubated with enzyme followed by substrate addition and measured after 30 mins by filter binding assay | Homo sapiens | 17 | assay format | Scientific Literature | ||
4. | ALA4771191 | B | Inhibition of N7-MTase activity of N-terminal His6-tagged SARS-CoV nsp14 expressed in Escherichia coli using GpppAC4 and [3H]SAM as substrate preincubated with enzyme followed by substrate addition and measured after 30 mins by filter binding assay | Severe acute respiratory syndrome-related coronavirus | 11 | assay format | Scientific Literature | ||
5. | ALA4771192 | A | Inhibition of N-terminal His6-tagged human RNA-N7 MTase expressed in Escherichia coli using GpppAC4 and [3H]SAM as substrate preincubated with enzyme followed by substrate addition and measured after 30 mins by filter binding assay | Homo sapiens | 10 | assay format | Scientific Literature | ||
6. | ALA4771193 | B | Binding affinity to SARS-CoV nsp14 assessed as increase in melting temperature at 1 mM at pH 7.5 by differential scanning fluorimetry | Severe acute respiratory syndrome-related coronavirus | 11 | assay format | Scientific Literature | ||
7. | ALA4771195 | B | Binding affinity to SARS-CoV nsp14 assessed as melting temperature at 1 mM at pH 7.5 by differential scanning fluorimetry | Severe acute respiratory syndrome-related coronavirus | 9 | assay format | Scientific Literature | ||
8. | ALA4771197 | B | Inhibition of 2'-O-MTase activity of SARS-CoV nsp10/16 complex at 200 uM | Severe acute respiratory syndrome-related coronavirus | 16 | assay format | Scientific Literature | ||
9. | ALA4771198 | B | Inhibition of 2'-O-methyltransferase in Zika virus at 200 uM | Zika virus | 16 | assay format | Scientific Literature | ||
10. | ALA4771199 | B | Inhibition of 2'-O-methyltransferase in West-Nile virus at 200 uM | West Nile virus | 16 | assay format | Scientific Literature | ||
11. | ALA4771200 | B | Inhibition of 2'-O-methyltransferase in Dengue virus at 200 uM | Dengue virus | 16 | assay format | Scientific Literature | ||
12. | ALA4771201 | B | Inhibition of 2'-O-methyltransferase activity of Vaccinia virus VP39 at 200 uM | Vaccinia virus | 16 | assay format | Scientific Literature |