# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4772722 | P | Aqueous stability of compound | 25 | small-molecule physicochemical format | Scientific Literature | |||
2. | ALA4772723 | F | Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced IL6 release at 5 to 10 uM preincubated for 1 hr followed by LPS stimulation and measured after 24 hrs by ELISA | Mus musculus | 23 | cell-based format | Scientific Literature | ||
3. | ALA4772725 | A | Cytotoxicity against mouse RAW264.7 cells assessed as reduction cell viability at 5 to 50 uM measured after 24 hrs by Celltiter-glo assay | Mus musculus | 4 | cell-based format | Scientific Literature | ||
4. | ALA4772727 | F | Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced TNFalpha release at 5 to 10 uM preincubated for 1 hr followed by LPS stimulation and measured after 4 hrs by ELISA | Mus musculus | 15 | cell-based format | Scientific Literature | ||
5. | ALA4772729 | B | Inhibition of LPS-induced NF-kappaB activity in mouse RAW-Blue cells assessed as reduction in SEAP reporter activity at 5 to 20 uM preincubated for 1 hr followed by LPS stimulation and measured after 8 hrs by SEAP reporter gene assay | Mus musculus | 4 | cell-based format | Scientific Literature | ||
6. | ALA4772730 | B | Inhibition of LPS-induced NF-kappaB activity in mouse RAW-Blue cells assessed as reduction in SEAP reporter activity at 5 to 20 uM preincubated for 1 hr followed by LPS stimulation and measured after 24 hrs by SEAP reporter gene assay | Mus musculus | 4 | cell-based format | Scientific Literature | ||
7. | ALA4772731 | B | Activation of NF-kappaB activity in mouse RAW-Blue cells assessed as increase in SEAP reporter activity at 5 to 20 uM measured after 24 hrs by SEAP reporter gene assay | Mus musculus | 3 | cell-based format | Scientific Literature | ||
8. | ALA4772732 | B | Activation of NF-kappaB activity in mouse RAW-Blue cells assessed as increase in SEAP reporter activity at 30 uM measured after 24 hrs in presence of p38 inhibitor SB203580 by SEAP reporter gene assay | Mus musculus | 3 | cell-based format | Scientific Literature | ||
9. | ALA4772733 | A | GSH reactivity in PBS assessed as GSH adduct formation measured up to 24 hrs by HPLC-MS analysis | 3 | assay format | Scientific Literature | |||
10. | ALA4772736 | A | GSH reactivity in PBS assessed as isomerization measured up to 24 hrs by HPLC-MS analysis | 2 | assay format | Scientific Literature | |||
11. | ALA4772737 | A | GSH reactivity in PBS assessed as GSH adduct formation measured up to 8 hrs by HPLC-MS analysis | 4 | assay format | Scientific Literature | |||
12. | ALA4772739 | A | GSH reactivity in PBS assessed as GSH adduct formation by measuring half life by HPLC-MS analysis | 4 | assay format | Scientific Literature | |||
13. | ALA4772741 | A | GSH reactivity assessed as GSH adduct formation by measuring reduction in intracellular GSH level at 5 to 20 uM measured after 2 hrs by cell-based monochlorobimane fluorometric method | 4 | cell-based format | Scientific Literature | |||
14. | ALA4772743 | A | GSH reactivity assessed as GSH adduct formation by measuring reduction in intracellular GSH level at 5 uM measured after 24 hrs by cell-based monochlorobimane fluorometric method | 1 | cell-based format | Scientific Literature | |||
15. | ALA4772744 | B | Inhibition of LPS-induced AKT activation in mouse RAW264.7 cells assessed as reduction in AKT phosphorylation measured after 4 hrs by Western blot analysis | Mus musculus | 1 | cell-based format | Scientific Literature | ||
16. | ALA4772745 | A | Effect on total AKT expression in LPS-induced mouse RAW264.7 cells at 5 to 20 uM measured after 4 hrs by Western blot analysis | Mus musculus | 1 | tissue-based format | Scientific Literature | ||
17. | ALA4772746 | B | Inhibition of LPS-induced ERK1/2 activation in mouse RAW264.7 cells assessed as reduction in ERK1/2 phosphorylation at 20 uM measured after 4 hrs by Western blot analysis | Mus musculus | 1 | cell-based format | Scientific Literature | ||
18. | ALA4772747 | B | Inhibition of DNA binding activity of human NF-kappaB p50 measured after 1 hr by ELISA | Homo sapiens | 1 | single protein format | Scientific Literature | ||
19. | ALA4772748 | B | Binding affinity to human NF-kappaB p50 assessed as compound-adduct formation by MALDI-TOF analysis | Homo sapiens | 1 | single protein format | Scientific Literature | ||
20. | ALA4772750 | A | Cytotoxicity against human L02 cells assessed as reduction in cell viability measured after 24 hrs by Celltiter-glo assay | Homo sapiens | 2 | cell-based format | Scientific Literature |