| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA4680948 | B | Binding affinity to Mycobacterium tuberculosis CysM expressed in Escherichia coli strain BL21 (DE3) incubated for 5 mins by PLP fluorescence-based screening assay | Mycobacterium tuberculosis | 15 | assay format | Scientific Literature | ||
| 2. | ALA4680949 | B | Inhibition of Mycobacterium tuberculosis CysM expressed in Escherichia coli strain BL21 (DE3) assessed as apparent first-order rate constant for the formation of the alpha-aminoacrylate intermediate at 100 uM incubated for 5 mins in presence of OPS by spectrophotometry | Mycobacterium tuberculosis | 19 | assay format | Scientific Literature | ||
| 3. | ALA4680950 | B | Inhibition of Mycobacterium tuberculosis CysM expressed in Escherichia coli strain BL21 (DE3) assessed as reduction in amino-acrylate intermediate formation by measuring residual enzyme activity at 100 uM incubated for 5 mins in presence of OPS by spectrophotometry | Mycobacterium tuberculosis | 10 | assay format | Scientific Literature | ||
| 4. | ALA4680951 | F | Antimycobacterial activity against Mycobacterium tuberculosis H37Rv assessed as inhibition of microbial growth incubated for 5 days by alamar blue assay | Mycobacterium tuberculosis | 10 | organism-based format | Scientific Literature | ||
| 5. | ALA4680957 | A | Cytotoxicity in human SH-SY5Y cells assessed as reduction in cell viability at 10 uM incubated for 72 hrs by Cell-titer-Glo assay | Homo sapiens | 6 | cell-based format | Scientific Literature | ||
| 6. | ALA4680958 | A | Cytotoxicity in human HepG2 cells assessed as reduction in cell viability at 10 uM incubated for 72 hrs by Cell-titer-Glo assay | Homo sapiens | 6 | cell-based format | Scientific Literature | ||
| 7. | ALA4680959 | A | Cytotoxicity in mouse J774.A1 cells assessed as reduction in cell viability at 10 uM incubated for 72 hrs by Cell-titer-Glo assay | Mus musculus | 6 | cell-based format | Scientific Literature | ||
| 8. | ALA4680960 | A | Cytotoxicity in human THP-1 cells assessed as reduction in cell viability at 10 uM incubated for 72 hrs by Cell-titer-Glo assay | Homo sapiens | 6 | cell-based format | Scientific Literature | ||
| 9. | ALA4680961 | B | Binding affinity to Mycobacterium tuberculosis CysK1 by PLP fluorescence-based screening assay | Mycobacterium tuberculosis | 2 | assay format | Scientific Literature | ||
| 10. | ALA4680962 | B | Selectivity index, ratio of Kd for binding affinity to Mycobacterium tuberculosis CysK1 to Kd for binding affinity to Mycobacterium tuberculosis CysM expressed in Escherichia coli strain BL21 (DE3) | Mycobacterium tuberculosis | 2 | assay format | Scientific Literature | ||
| 11. | ALA4680963 | B | Binding affinity to Mycobacterium tuberculosis CysK2 by PLP fluorescence-based screening assay | Mycobacterium tuberculosis | 2 | assay format | Scientific Literature | ||
| 12. | ALA4680964 | B | Selectivity index, ratio of Kd for binding affinity to Mycobacterium tuberculosis CysK2 to Kd for binding affinity to Mycobacterium tuberculosis CysM expressed in Escherichia coli strain BL21 (DE3) | Mycobacterium tuberculosis | 2 | assay format | Scientific Literature | ||
| 13. | ALA4680965 | B | Binding affinity to human CBS by PLP fluorescence-based screening assay | Homo sapiens | 2 | single protein format | Scientific Literature | ||
| 14. | ALA5262985 | B | Inhibition of hexahistidine-tagged recombinant Mycobacterium tuberculosis CysK1 expressed in Escherichia coli BL21(DE3) assessed as dissociation constant using PLP as substrate incubated for 5 mins by fluorescence analysis | Mycobacterium tuberculosis | 5 | assay format | Scientific Literature | ||
| 15. | ALA5262987 | B | Inhibition of hexahistidine-tagged recombinant Mycobacterium tuberculosis CysK2 expressed in Escherichia coli BL21(DE3) assessed as inhibition constant using O-acetyl-L-serine as substrate by Lineweaver-Burk plot analysis | Mycobacterium tuberculosis | 1 | assay format | Scientific Literature | ||
| 16. | ALA5262988 | B | Inhibition of hexahistidine-tagged recombinant Mycobacterium tuberculosis CysK2 expressed in Escherichia coli BL21(DE3) assessed as amount of S-sulfocysteine produced using O-acetyl-L-serine as substrate incubated for 30 mins by acid-ninhydrin based spectrophotometric assay | Mycobacterium tuberculosis | 12 | assay format | Scientific Literature | ||
| 17. | ALA5262989 | B | Inhibition of hexahistidine-tagged recombinant Mycobacterium tuberculosis CysK1 expressed in Escherichia coli BL21(DE3) assessed as amount of cysteine produced using O-acetyl-L-serine as substrate incubated for 12 mins by acid-ninhydrin based spectrophotometric assay | Mycobacterium tuberculosis | 12 | assay format | Scientific Literature | ||
| 18. | ALA5262990 | B | Inhibition of hexahistidine-tagged recombinant Mycobacterium tuberculosis CysK2 expressed in Escherichia coli BL21(DE3) assessed as dissociation constant using PLP as substrate incubated for 5 mins by fluorescence analysis | Mycobacterium tuberculosis | 7 | assay format | Scientific Literature |
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