# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4670803 | B | Inhibition of TEAD4 in HEK293 cells assessed as reduction in ANKRD1 mRNA expression at 1 uM measured after 6 hrs by SYBR green dye based RT-PCR analysis relative to control | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
2. | ALA4670805 | B | Binding affinity to nonacylated mouse TEAD4 at 0.5 mM by 1H-15N HSQC spectra analysis | Mus musculus | 1 | single protein format | Scientific Literature | ||
3. | ALA4670810 | B | Binding affinity to nonacylated N-terminal His6-tagged human TEAD4 (217 to 434 residues) expressed in Escherichia coli BL21-CodonPlus (DE3)-RIPL cells assessed as inhibition of protein-FAM-YAP (50 to 100 residues) interaction preincubated for 10 mins followed by FAM YAP addition and measured after 1 hr by fluorescence assay | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
4. | ALA4670813 | B | Inhibition of CPM binding to N-terminal His6-tagged human TEAD4 (217 to 434 residues) expressed in Escherichia coli BL21-CodonPlus (DE3)-RIPL cells preincubated for 10 mins followed by CPM addition and measured after 1 hr by fluorescence assay | Homo sapiens | 38 | cell-based format | Scientific Literature | ||
5. | ALA4670814 | B | Inhibition of CPM binding to N-terminal His6-tagged human TEAD4 (217 to 434 residues) expressed in Escherichia coli BL21-CodonPlus (DE3)-RIPL cells at 12.5 uM preincubated for 10 mins followed by CPM addition and measured after 1 hr by fluorescence assay relative to control | Homo sapiens | 34 | cell-based format | Scientific Literature | ||
6. | ALA4670815 | B | Inhibition of FITC-labeled palmitate tracer binding to N-terminal His6-tagged human TEAD4 (217 to 434 residues) expressed in Escherichia coli BL21-CodonPlus (DE3)-RIPL cells preincubated for 10 mins followed by FITC-labeled palmitate tracer addition and measured after 1 hr by fluorescence assay | Homo sapiens | 31 | cell-based format | Scientific Literature | ||
7. | ALA4670816 | B | Selectivity ratio of apparent IC50 for inhibition of CPM binding to N-terminal His6-tagged human TEAD4 (217 to 434 residues) expressed in Escherichia coli BL21-CodonPlus (DE3)-RIPL cells to IC50 for inhibition of FITC-labeled palmitate tracer binding to N-terminal His6-tagged human TEAD4 (217 to 434 residues) expressed in Escherichia coli BL21-CodonPlus (DE3)-RIPL cells | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
8. | ALA4670817 | B | Binding affinity to nonacylated N-terminal His6-tagged human TEAD4 (217 to 434 residues) expressed in Escherichia coli BL21-CodonPlus (DE3)-RIPL cells assessed as melting temperature at 80 uM after 10 mins by SYPRO orange dye-based differential scanning fluorimetry method (Rvb = 41 degreeC) | Homo sapiens | 4 | cell-based format | Scientific Literature | ||
9. | ALA4670818 | B | Binding affinity to nonacylated N-terminal His6-tagged human TEAD4 (217 to 434 residues) expressed in Escherichia coli BL21-CodonPlus (DE3)-RIPL cells at 8 equiv after 1 hr by ESI-MS spectrophotometric analysis | Homo sapiens | 2 | cell-based format | Scientific Literature |