# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4683919 | Functional | Induction of cell cycle arrest in human HCC827 cells assessed as accumulation in G1 phase at 1 uM incubated for 32 hrs by PI staining based flow cytometry analysis (Rvb = 50.48 %) | Homo sapiens | 4 | ALA4680163 | cell-based format | Scientific Literature | |
2. | ALA4683920 | Functional | Antiproliferative activity against human HCC827 cells harboring EGFRDel19 mutant assessed as reduction in cell viability incubated for 72 hrs by MTT assay | Homo sapiens | 16 | ALA4680163 | cell-based format | Scientific Literature | |
3. | ALA4683921 | Functional | Antiproliferative activity against human NCI-H1975 cells harboring EGFR L858/T790M mutant assessed as reduction in cell viability incubated for 72 hrs by MTT assay | Homo sapiens | 16 | ALA4680163 | cell-based format | Scientific Literature | |
4. | ALA4683922 | Functional | Antiproliferative activity against human A549 cells harboring EGFR assessed as reduction in cell viability incubated for 72 hrs by MTT assay | Homo sapiens | 16 | ALA4680163 | cell-based format | Scientific Literature | |
5. | ALA4683923 | Functional | Antiproliferative activity against human A-431 cells harboring EGFR assessed as reduction in cell viability incubated for 72 hrs by MTT assay | Homo sapiens | 17 | ALA4680163 | cell-based format | Scientific Literature | |
6. | ALA4683924 | Binding | Protac activity at E3 ubiquitin ligase/EGFR in human HCC827 cells assessed as increase in degradation of EGFR incubated for 48 hrs by Western blot analysis | Homo sapiens | 2 | ALA4680163 | cell-based format | Scientific Literature | |
7. | ALA4683925 | Binding | Protac activity at E3 ubiquitin ligase/EGFR in human HCC827 cells assessed as increase in degradation of EGFR measured upto 96 hrs by Western blot analysis | Homo sapiens | 2 | ALA4680163 | cell-based format | Scientific Literature | |
8. | ALA4683927 | Binding | Protac activity at E3 ubiquitin ligase/EGFR in human HCC827 cells assessed as increase in degradation of EGFR at 100 nM incubated for 72 hrs by Western blot analysis relative to control | Homo sapiens | 1 | ALA4680163 | cell-based format | Scientific Literature | |
9. | ALA4683928 | Binding | Inhibition of EGFR in human HCC827 cells assessed as reduction in EGFR phosphorylation incubated for 36 hrs by Western blot analysis | Homo sapiens | 3 | ALA4680163 | cell-based format | Scientific Literature | |
10. | ALA4683929 | Binding | Inhibition of EGFR in human HCC827 cells assessed as reduction in Akt phosphorylation incubated for 36 hrs by Western blot analysis | Homo sapiens | 4 | ALA4680163 | cell-based format | Scientific Literature | |
11. | ALA4683930 | Binding | Protac activity at E3 ubiquitin ligase/EGFR in human HCC827 cells assessed as increase in degradation of EGFR at 0.1 uM pretreated with (1-(6-(5-(4-(dimethylamino)piperidin-1-yl)pyridin-2-ylamino)-2-(4-fluorophenylamino)pyrido[3,4-d]pyrimidin-4-yl)piperidin-4-yl)methanol and incubated for 6 hrs followed by compound addition and measured after 32 hrs by Western blot analysis | Homo sapiens | 2 | ALA4680163 | cell-based format | Scientific Literature | |
12. | ALA4683932 | Binding | Protac activity at E3 ubiquitin ligase/EGFR in human HCC827 cells assessed as increase in degradation of EGFR at 100 nM pretreated with VHL-L for 5 hrs followed by compound addition and measured after 32 hrs by Western blot analysis | Homo sapiens | 1 | ALA4680163 | cell-based format | Scientific Literature | |
13. | ALA4683933 | Binding | Protac activity at E3 ubiquitin ligase/EGFR in human HCC827 cells assessed as increase in degradation of EGFR at 1 to 10 uM pretreated with MG-132 for 6 hrs followed by compound addition and measured after 30 hrs by Western blot analysis | Homo sapiens | 2 | ALA4680163 | cell-based format | Scientific Literature | |
14. | ALA4683934 | Binding | Protac activity at E3 ubiquitin ligase/EGFR in human HCC827 cells assessed as increase in degradation of EGFR at 1 to 10 uM pretreated with MLN-4924 for 6 hrs followed by compound addition and measured after 30 hrs by Western blot analysis | Homo sapiens | 2 | ALA4680163 | cell-based format | Scientific Literature | |
15. | ALA4683935 | Binding | Protac activity at E3 ubiquitin ligase/EGFR in human NCI-H1975 cells harboring EGFR T790M mutant assessed as increase in degradation of EGFR at 0.1 to 10 uM incubated for 48 hrs by Western blot analysis | Homo sapiens | 2 | ALA4680163 | cell-based format | Scientific Literature | |
16. | ALA4683936 | Binding | Protac activity at E3 ubiquitin ligase/EGFR in human A549 cells harboring wild type EGFR assessed as increase in degradation of EGFR at 0.1 to 10 uM incubated for 48 hrs by Western blot analysis | Homo sapiens | 2 | ALA4680163 | cell-based format | Scientific Literature | |
17. | ALA4683937 | Functional | Induction of apoptosis in human HCC827 cells assessed as viable cells at 0.1 uM incubated for 32 hrs by annexin V-FITC and PI staining based flow cytometry analysis (Rvb = 76.79 %) | Homo sapiens | 4 | ALA4680163 | cell-based format | Scientific Literature | |
18. | ALA4683938 | Functional | Induction of apoptosis in human HCC827 cells assessed as early apoptotic cells at 0.1 uM incubated for 32 hrs by annexin V-FITC and PI staining based flow cytometry analysis (Rvb = 2.31 %) | Homo sapiens | 4 | ALA4680163 | cell-based format | Scientific Literature | |
19. | ALA4683939 | Functional | Induction of apoptosis in human HCC827 cells assessed as late apoptotic cells at 0.1 uM incubated for 32 hrs by annexin V-FITC and PI staining based flow cytometry analysis (Rvb = 14.23 %) | Homo sapiens | 4 | ALA4680163 | cell-based format | Scientific Literature | |
20. | ALA4683940 | Functional | Induction of apoptosis in human HCC827 cells assessed as necrotic cells at 0.1 uM incubated for 32 hrs by annexin V-FITC and PI staining based flow cytometry analysis (Rvb = 6.68 %) | Homo sapiens | 4 | ALA4680163 | cell-based format | Scientific Literature |