#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA4710535	Binding	Inhibition of human ANO1 channel expressed in HEK293 cells at 10 uM incubated for 10 mins by FLIPR assay relative to control	Homo sapiens	36	ALA4706565	cell-based format	Scientific Literature
2.	ALA4710536	Binding	Inhibition of human ANO1 channel expressed in HEK293 cells incubated for 10 mins by FLIPR assay	Homo sapiens	10	ALA4706565	cell-based format	Scientific Literature
3.	ALA4710537	Binding	Inhibition of GFP-fused mouse ANO1 channel expressed in HEK293A cells assessed as inhibition of ATP-induced channel current amplitude at membrane potential of +80 mV by patch clamp electrophysiology assay	Mus musculus	11	ALA4706565	cell-based format	Scientific Literature
4.	ALA4710542	Functional	Inhibition of anchorage-independent colony formation in human U251 cells at 10 uM incubated for 14 days by crystal violet staining based soft agar colony formation assay	Homo sapiens	6	ALA4706565	cell-based format	Scientific Literature
5.	ALA4710543	Functional	Inhibition of colony formation in human U251 cells incubated for 14 days by crystal violet dye based assay	Homo sapiens	2	ALA4706565	cell-based format	Scientific Literature
6.	ALA4710544	Functional	Inhibition of anchorage-independent colony formation in human U251 cells at 100 uM incubated for 14 days by crystal violet staining based soft agar colony formation assay	Homo sapiens	6	ALA4706565	cell-based format	Scientific Literature
7.	ALA4710545	Functional	Inhibition of cell migration in human U251 cells at 10 uM incubated for 18 hrs by wound healing assay	Homo sapiens	6	ALA4706565	cell-based format	Scientific Literature
8.	ALA4710546	Functional	Inhibition of cell invasion in human U251 cells at 10 uM incubated for 48 hrs by Boyden chamber method	Homo sapiens	6	ALA4706565	cell-based format	Scientific Literature
9.	ALA4710547	Functional	Upregulation of E-cadherin protein expression in human U251 cells incubated for 18 hrs by Western blot analysis	Homo sapiens	2	ALA4706565	cell-based format	Scientific Literature
10.	ALA4710548	Binding	Induction of ANO1 degradation in human U251 cells at 10 uM preincubated with compound for 6 hrs followed by treatment with proteasome inhibitor, MG132 for 18 hrs by western blot analysis	Homo sapiens	2	ALA4706565	cell-based format	Scientific Literature
11.	ALA4710549	Functional	Antiproliferative activity against human U251 cells overexpressing ANO1 assessed as reduction in cell viability measured after 72 hrs by Celltiter-glo assay	Homo sapiens	10	ALA4706565	cell-based format	Scientific Literature
12.	ALA4710550	Functional	Antiproliferative activity against human U87MG cells overexpressing ANO1 assessed as reduction in cell viability measured after 72 hrs by Celltiter-glo assay	Homo sapiens	6	ALA4706565	cell-based format	Scientific Literature
13.	ALA4710551	Functional	Antiproliferative activity against human U138 cells overexpressing ANO1 assessed as reduction in cell viability measured after 72 hrs by Celltiter-glo assay	Homo sapiens	6	ALA4706565	cell-based format	Scientific Literature
14.	ALA4710552	Functional	Inhibition of colony formation in human U251 cells at 10 uM incubated for 14 days by crystal violet dye based assay	Homo sapiens	6	ALA4706565	cell-based format	Scientific Literature
15.	ALA4710553	Functional	Inhibition of colony formation in human U251 cells at 3 uM incubated for 14 days by crystal violet dye based assay	Homo sapiens	2	ALA4706565	cell-based format	Scientific Literature
16.	ALA4710555	Functional	Inhibition of anchorage-independent colony formation in human U251 cells incubated for 14 days by crystal violet staining based soft agar colony formation assay	Homo sapiens	2	ALA4706565	cell-based format	Scientific Literature
17.	ALA4710556	Functional	Inhibition of cell migration in human U251 cells at 5 uM incubated for 18 hrs by wound healing assay	Homo sapiens	2	ALA4706565	cell-based format	Scientific Literature
18.	ALA4710557	Functional	Inhibition of cell migration in human U251 cells incubated for 18 hrs by wound healing assay	Homo sapiens	2	ALA4706565	cell-based format	Scientific Literature
19.	ALA4710558	Binding	Induction of ANO1 degradation in human U251 cells at 10 uM incubated for 24 hrs by western blot analysis	Homo sapiens	2	ALA4706565	cell-based format	Scientific Literature
20.	ALA4710559	Binding	Inhibition of GFP-fused mouse ANO1 channel expressed in HEK293A cells assessed as inhibition of current amplitude at 10 uM at membrane potential of +80 mV by patch clamp electrophysiology assay	Mus musculus	2	ALA4706565	cell-based format	Scientific Literature