# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4731317 | A | Displacement of fluorescent probe SLFb-2PEG-TAMRA from N-terminal 6His-SUMO-tagged human full length FKBP12 expressed in Escherichia coli BL21 (DE3) cells measured after 1 hr by fluorescence polarisation assay | Homo sapiens | 16 | cell-based format | Scientific Literature | ||
2. | ALA4731318 | B | Binding affinity to N-terminal 6His-SUMO-tagged Plasmodium falciparum FKBP35 binding domain (FBD35) (Q5-A128) expressed in Escherichia coli BL21 (DE3) cells assessed as change in melting temperature at 40 uM by differential scanning colorimetric analysis | Plasmodium falciparum | 19 | cell-based format | Scientific Literature | ||
3. | ALA4731319 | B | Displacement of fluorescent probe SLFb-2PEG-TAMRA from N-terminal 6His-SUMO-tagged Plasmodium falciparum FKBP35 binding domain (FBD35) (Q5-A128) expressed in Escherichia coli BL21 (DE3) cells measured after 1 hr by fluorescence polarisation assay | Plasmodium falciparum | 16 | cell-based format | Scientific Literature | ||
4. | ALA4731320 | A | Binding affinity to N-terminal 6His-SUMO-tagged human full length FKBP12 expressed in Escherichia coli BL21 (DE3) cells assessed as change in melting temperature at 40 uM by differential scanning colorimetric analysis | Homo sapiens | 19 | cell-based format | Scientific Literature | ||
5. | ALA4731321 | P | Aqueous solubility of compound in PBS at pH 7.4 | 7 | small-molecule physicochemical format | Scientific Literature | |||
6. | ALA4731327 | B | Covalent binding affinity to N-terminal 6His-SUMO-tagged Plasmodium falciparum FKBP35 binding domain (FBD35) (Q5-A128) expressed in Escherichia coli BL21 (DE3) cells assessed as reaction half-life for FKBP35 consumption at 2250 nM incubated for 24 hrs by LC-MS analysis | Plasmodium falciparum | 2 | cell-based format | Scientific Literature | ||
7. | ALA4731329 | F | Antiplasmodial activity against Plasmodium falciparum NF54 assessed as growth inhibition incubated for 72 hrs by renilla luciferase reporter gene assay | Plasmodium falciparum | 5 | organism-based format | Scientific Literature | ||
8. | ALA4731331 | B | Displacement of fluorescent tracer Rap-Gly-BDP from nanoluciferse-tagged Plasmodium falciparum FKBP35 expressed in HEK293T cells at 0.001 to 100 uM incubated for 2 hrs by NanoBRET assay | Plasmodium falciparum | 7 | cell-based format | Scientific Literature | ||
9. | ALA4731332 | A | Displacement of fluorescent tracer Rap-Gly-BDP from nanoluciferse-tagged human FKBP12 expressed in HEK293T cells at 0.001 to 100 uM incubated for 2 hrs by NanoBRET assay | Homo sapiens | 7 | cell-based format | Scientific Literature | ||
10. | ALA4731333 | A | Stability of the compound in human plasma at 1 uM incubated for 5 hrs by UPLC-MS/MS analysis | Homo sapiens | 2 | cell-free format | Scientific Literature | ||
11. | ALA4731334 | A | Stability of the compound in mouse plasma at 1 uM incubated for 5 hrs by UPLC-MS/MS analysis | Mus musculus | 2 | cell-free format | Scientific Literature |