# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4771737 | B | Inhibition of transport activity of human GLUT5 expressed in hexose transporter deficient VW4000 yeast cells assessed as reduction in transporter-mediated [14C]-fructose uptake into cells at 100 uM measured after 10 mins by scintillation counting method | Homo sapiens | 23 | ALA4765368 | cell-based format | Scientific Literature | |
2. | ALA4771738 | B | Inhibition of transport activity of human GLUT1 expressed in hexose transporter deficient VW4000fgy1 yeast cells assessed as reduction in transporter-mediated [14C]-hexose uptake into cells at 100 uM measured after 10 mins by scintillation counting method relative to control | Homo sapiens | 3 | ALA4765368 | cell-based format | Scientific Literature | |
3. | ALA4771739 | B | Inhibition of transport activity of human GLUT4 expressed in hexose transporter deficient W4000fgy1 erg4 yeast cells assessed as reduction in transporter-mediated [14C]-hexose uptake into cells at 100 uM measured after 10 mins by scintillation counting method relative to control | Homo sapiens | 3 | ALA4765368 | cell-based format | Scientific Literature | |
4. | ALA4771740 | B | Inhibition of transport activity of human GLUT1 expressed in hexose transporter deficient VW4000fgy1 yeast cells assessed as reduction in transporter-mediated [14C]-hexose uptake into cells measured after 10 mins by scintillation counting method | Homo sapiens | 3 | ALA4765368 | cell-based format | Scientific Literature | |
5. | ALA4771741 | B | Inhibition of transport activity of human GLUT4 expressed in hexose transporter deficient W4000fgy1 erg4 yeast cells assessed as reduction in transporter-mediated [14C]-hexose uptake into cells measured after 10 mins by scintillation counting method | Homo sapiens | 3 | ALA4765368 | cell-based format | Scientific Literature | |
6. | ALA4771744 | F | Cytotoxicity against human CCRF-CEM cells assessed as reduction in cell viability measured after 48 hrs by Hoechst 33342/propidium iodide staining based imaging analysis | Homo sapiens | 7 | ALA4765368 | cell-based format | Scientific Literature | |
7. | ALA4771745 | F | Cytotoxicity against human K562 cells assessed as reduction in cell viability measured after 48 hrs by Hoechst 33342/propidium iodide staining based imaging analysis | Homo sapiens | 7 | ALA4765368 | cell-based format | Scientific Literature | |
8. | ALA4771746 | F | Cytotoxicity against human KCL-22 cells assessed as reduction in cell viability measured after 48 hrs by Hoechst 33342/propidium iodide staining based imaging analysis | Homo sapiens | 7 | ALA4765368 | cell-based format | Scientific Literature | |
9. | ALA4771747 | F | Cytotoxicity against human MDA-MB-231 cells assessed as reduction in cell viability measured after 48 hrs by Hoechst 33342/propidium iodide staining based imaging analysis | Homo sapiens | 7 | ALA4765368 | cell-based format | Scientific Literature | |
10. | ALA4771748 | A | Cytotoxicity against human HS27 cells assessed as reduction in cell viability measured after 48 hrs by Hoechst 33342/propidium iodide staining based imaging analysis | Homo sapiens | 7 | ALA4765368 | cell-based format | Scientific Literature | |
11. | ALA4771750 | U | Selectivity index, ratio of CC50 for human HS27 cells to CC50 for human CCRF-CEM cells | Homo sapiens | 2 | ALA4765368 | assay format | Scientific Literature | |
12. | ALA4771751 | B | Inhibition of recombinant human His6/SUMO-tagged HDAC8 expressed in Escherichia coli (BL21) DE3 pLysS assessed as residual activity at 50 uM using Boc-Lys(trifluoroacetyl)-AMC as substrate preincubated for 60 mins followed by substrate addition and measured after 60 mins by fluorescence assay relative to control | Homo sapiens | 24 | ALA4765368 | assay format | Scientific Literature | |
13. | ALA4771753 | B | Inhibition of recombinant human N-terminal GST-tagged HDAC4 (627 to end residues) expressed in baculovirus infected Sf9 insect cells assessed as residual activity at 50 uM using Boc-Lys(trifluoroacetyl)-AMC as substrate preincubated for 60 mins followed by substrate addition and measured after 60 mins by fluorescence assay relative to control | Homo sapiens | 24 | ALA4765368 | cell-based format | Scientific Literature | |
14. | ALA4771754 | F | Induction of apoptosis in human CCRF-CEM cells assessed as late apoptotic cells at CC50 measured after 24 hrs by AnnexinV-FITC/propidium iodide staining based flow cytometry analysis relative to control | Homo sapiens | 1 | ALA4765368 | cell-based format | Scientific Literature | |
15. | ALA4771755 | F | Induction of apoptosis in human CCRF-CEM cells assessed as early apoptotic cells at CC50 measured after 24 hrs by AnnexinV-FITC/propidium iodide staining based flow cytometry analysis relative to control | Homo sapiens | 1 | ALA4765368 | cell-based format | Scientific Literature | |
16. | ALA4771756 | F | Induction of apoptosis in human CCRF-CEM cells assessed as live cells at CC50 measured after 24 hrs by AnnexinV-FITC/propidium iodide staining based flow cytometry analysis (Rvb = 92.15%) | Homo sapiens | 1 | ALA4765368 | cell-based format | Scientific Literature | |
17. | ALA4771757 | F | Induction of apoptosis in human CCRF-CEM cells assessed as necrotic cells at CC50 measured after 24 hrs by AnnexinV-FITC/propidium iodide staining based flow cytometry analysis relative to control | Homo sapiens | 1 | ALA4765368 | cell-based format | Scientific Literature | |
18. | ALA4771758 | F | Cell cycle arrest in human CCRF-CEM cells assessed as increase in accumulation at sub-G0/G1 phase measured after 72 hrs by NIM-DAPI staining based flow cytometry | Homo sapiens | 1 | ALA4765368 | cell-based format | Scientific Literature | |
19. | ALA4771759 | F | Cell cycle arrest in human CCRF-CEM cells assessed as decrease in accumulation at G0/G1 phase measured after 72 hrs by NIM-DAPI staining based flow cytometry | Homo sapiens | 1 | ALA4765368 | cell-based format | Scientific Literature | |
20. | ALA4771760 | F | Cell cycle arrest in human CCRF-CEM cells assessed as decrease in accumulation at S phase measured after 72 hrs by NIM-DAPI staining based flow cytometry | Homo sapiens | 1 | ALA4765368 | cell-based format | Scientific Literature |