#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA4670893	B	Binding affinity to BTN3A1 in human PBMC-derived Vgamma9/Vdelta2 T cells assessed as induction of Vgamma9/Vdelta2 T cell mediated T-24 cell lysis co-expressing GFP at 10 pM preincubated 4 hrs followed by compound washout and subsequent Vgamma9Vdelta2 T cell addition at 8:1 effector/target ratio and measured after 18 hrs by fluorescence based assay	Homo sapiens	5		tissue-based format	Scientific Literature
2.	ALA4670894	B	Binding affinity to BTN3A1 in human PBMC-derived Vgamma9/Vdelta2 T cells assessed as induction of Vgamma9/Vdelta2 T cell mediated T-24 cell lysis co-expressing GFP at 10 pM preincubated 4 hrs followed by compound washout and subsequent Vgamma9Vdelta2 T cell addition at 1:1 effector/target ratio and measured after 18 hrs by fluorescence based assay	Homo sapiens	5		tissue-based format	Scientific Literature
3.	ALA4670895	A	Cytotoxicity against human T-24 cells co-expressing GFP assessed as reduction in cell viability at 10 pM after 4 hrs by eFluor 780 fixable viability dye based fluorescence assay	Homo sapiens	5		cell-based format	Scientific Literature
4.	ALA4670897	B	Binding affinity to BTN3A1 in human PBMC-derived Vgamma9/Vdelta2 T cells assessed as increase in CD69/CD25 level incubated for overnight by flow cytometric analysis	Homo sapiens	10		tissue-based format	Scientific Literature