#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA4667958	Binding	Inhibition of recombinant Trypanosoma cruzi cruzain at 5 uM using Z-FR-AMC as substrate and measured at 12 sec interval for 5 mins without preincubation by fluorimetric analysis relative to control	Trypanosoma cruzi	70	ALA4665666	single protein format	Scientific Literature
2.	ALA4667959	Binding	Inhibition of recombinant Trypanosoma cruzi cruzain at 5 uM using Z-FR-AMC as substrate preincubated for 10 mins followed by substrate addition and measured at 12 sec interval for 5 mins by fluorimetric analysis relative to control	Trypanosoma cruzi	70	ALA4665666	single protein format	Scientific Literature
3.	ALA4667960	Binding	Inhibition of recombinant Trypanosoma brucei rhodesain expressed in Pichia pastoris at 5 uM using Z-FR-AMC as substrate and measured at 12 sec interval for 5 mins without preincubation by fluorimetric analysis relative to control	Trypanosoma brucei	70	ALA4665666	assay format	Scientific Literature
4.	ALA4667961	Binding	Inhibition of recombinant Trypanosoma brucei rhodesain expressed in Pichia pastoris at 5 uM using Z-FR-AMC as substrate preincubated for 10 mins followed by substrate addition and measured at 12 sec interval for 5 mins by fluorimetric analysis relative to control	Trypanosoma brucei	70	ALA4665666	assay format	Scientific Literature
5.	ALA4667968	Binding	Inhibition of recombinant Trypanosoma cruzi cruzain at 10 uM using Z-FR-AMC as substrate and measured at 12 sec interval for 5 mins without preincubation by fluorimetric analysis relative to control	Trypanosoma cruzi	14	ALA4665666	single protein format	Scientific Literature
6.	ALA4667969	Binding	Inhibition of recombinant Trypanosoma cruzi cruzain at 10 uM using Z-FR-AMC as substrate preincubated for 10 mins followed by substrate addition and measured at 12 sec interval for 5 mins by fluorimetric analysis relative to control	Trypanosoma cruzi	14	ALA4665666	single protein format	Scientific Literature
7.	ALA4667970	Binding	Inhibition of recombinant Trypanosoma brucei rhodesain expressed in Pichia pastoris at 10 uM using Z-FR-AMC as substrate and measured at 12 sec interval for 5 mins without preincubation by fluorimetric analysis relative to control	Trypanosoma brucei	14	ALA4665666	assay format	Scientific Literature
8.	ALA4667971	Binding	Inhibition of recombinant Trypanosoma brucei rhodesain expressed in Pichia pastoris at 10 uM using Z-FR-AMC as substrate preincubated for 10 mins followed by substrate addition and measured at 12 sec interval for 5 mins by fluorimetric analysis relative to control	Trypanosoma brucei	14	ALA4665666	assay format	Scientific Literature
9.	ALA4667980	Binding	Inhibition of recombinant Trypanosoma cruzi cruzain at 300 uM using Z-FR-AMC as substrate and measured at 12 sec interval for 5 mins without preincubation by fluorimetric analysis relative to control	Trypanosoma cruzi	2	ALA4665666	single protein format	Scientific Literature
10.	ALA4667981	Binding	Inhibition of recombinant Trypanosoma cruzi cruzain at 300 uM using Z-FR-AMC as substrate preincubated for 10 mins followed by substrate addition and measured at 12 sec interval for 5 mins by fluorimetric analysis relative to control	Trypanosoma cruzi	2	ALA4665666	single protein format	Scientific Literature
11.	ALA4667982	Binding	Inhibition of recombinant Trypanosoma brucei rhodesain expressed in Pichia pastoris at 300 uM using Z-FR-AMC as substrate and measured at 12 sec interval for 5 mins without preincubation by fluorimetric analysis relative to control	Trypanosoma brucei	2	ALA4665666	assay format	Scientific Literature
12.	ALA4667983	Binding	Inhibition of recombinant Trypanosoma brucei rhodesain expressed in Pichia pastoris at 300 uM using Z-FR-AMC as substrate preincubated for 10 mins followed by substrate addition and measured at 12 sec interval for 5 mins by fluorimetric analysis relative to control	Trypanosoma brucei	2	ALA4665666	assay format	Scientific Literature
13.	ALA4667988	Binding	Inhibition of recombinant Trypanosoma cruzi cruzain using Z-FR-AMC as substrate and measured at 12 sec interval for 5 mins without preincubation by fluorimetric analysis	Trypanosoma cruzi	4	ALA4665666	single protein format	Scientific Literature
14.	ALA4667990	Binding	Inhibition of recombinant Trypanosoma brucei rhodesain expressed in Pichia pastoris using Z-FR-AMC as substrate and measured at 12 sec interval for 5 mins without preincubation by fluorimetric analysis	Trypanosoma brucei	2	ALA4665666	assay format	Scientific Literature
15.	ALA4667992	Binding	Inhibition of recombinant Trypanosoma cruzi cruzain at 100 uM preincubated for 10 mins in presence of 0.1% Triton X-100 relative to control	Trypanosoma cruzi	4	ALA4665666	single protein format	Scientific Literature
16.	ALA4667993	Binding	Inhibition of recombinant Trypanosoma cruzi cruzain at 100 uM preincubated for 10 mins in presence of 0.01% Triton X-100 relative to control	Trypanosoma cruzi	4	ALA4665666	single protein format	Scientific Literature
17.	ALA4667994	Binding	Inhibition of recombinant Trypanosoma cruzi cruzain at 100 uM preincubated for 10 mins in absence of Triton X-100 relative to control	Trypanosoma cruzi	4	ALA4665666	single protein format	Scientific Literature
18.	ALA4668004	Binding	Inhibition of recombinant Trypanosoma cruzi cruzain at 100 uM preincubated with BSA for 5 mins followed by incubation with enzyme for 5 mins followed by substrate addition relative to control	Trypanosoma cruzi	4	ALA4665666	single protein format	Scientific Literature
19.	ALA4668005	Binding	Inhibition of recombinant Trypanosoma cruzi cruzain at 100 uM preincubated with enzyme for 5 mins followed by substrate addition in absence of BSA relative to control	Trypanosoma cruzi	4	ALA4665666	single protein format	Scientific Literature
20.	ALA4668013	Binding	Competitive inhibition of recombinant Trypanosoma cruzi cruzain by Lineweaver-Burk plot analysis	Trypanosoma cruzi	2	ALA4665666	single protein format	Scientific Literature