| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA4702207 | B | Binding affinity to C-terminal His-tagged recombinant human MIF expressed in Escherichia coli BL21 (DE3) at 100 nM by fluorescence assay | Homo sapiens | 2 | assay format | Scientific Literature | ||
| 2. | ALA4702212 | B | Inhibition of C-terminal His-tagged recombinant human MIF expressed in Escherichia coli BL21 (DE3) using 4-HPP as substrate preincubated for 10 mins followed by substrate addition by UV absorption spectrum analysis | Homo sapiens | 22 | assay format | Scientific Literature | ||
| 3. | ALA4702221 | B | Displacement of 4'-(7-Hydroxy-2-oxo-2H-chromen-3-yl)-[1,1'-biphenyl]-4-carboxylic acid from C-terminal His-tagged recombinant human MIF expressed in Escherichia coli BL21 (DE3) preincubated for 10 mins followed by probe addition and measured after 10 mins by fluorescence assay | Homo sapiens | 8 | assay format | Scientific Literature | ||
| 4. | ALA4702223 | B | Binding affinity to C-terminal His-tagged recombinant human MIF expressed in Escherichia coli BL21 (DE3) assessed as inhibition of MIF interaction with MBP-fused human CD74 up to 50 uM preincubated for 10 mins with CD74 followed by MIF addition and measured after 30 mins by ELISA | Homo sapiens | 2 | assay format | Scientific Literature |
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