#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA4775281	B	Inhibition of NLRP3 in PMA-stimulated human THP-1 cells assessed as reduction in IL-1beta release at 20 uM pre-treated for 24 hrs followed by stimulation with LPS for 3 hrs and ATP for 1 hr by ELISA analysis	Homo sapiens	32		tissue-based format	Scientific Literature
2.	ALA4775282	B	Inhibition of NLRP3 in PMA-stimulated human THP-1 cells assessed as reduction in IL-1beta release at 50 uM pre-treated for 24 hrs followed by stimulation with LPS for 3 hrs and ATP for 1 hr by ELISA analysis	Homo sapiens	32		tissue-based format	Scientific Literature
3.	ALA4775285	A	Cytotoxicity in human THP-1 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	Homo sapiens	29		cell-based format	Scientific Literature
4.	ALA4775286	B	Inhibition of NLRP3 in PMA-stimulated human THP-1 cells assessed as reduction in IL-1beta release pre-treated for 24 hrs followed by stimulation with LPS for 3 hrs and ATP for 1 hr by ELISA analysis	Homo sapiens	6		tissue-based format	Scientific Literature
5.	ALA4775287	B	Inhibition of NLRP3 inflammasome activation in PMA-stimulated human THP-1 cells assessed as reduction in cleaved IL-1beta secretion at 15 to 45 uM pre-treated for 24 hrs followed by stimulation with LPS for 3 hrs and ATP for 1 hr by Western blot analysis	Homo sapiens	1		tissue-based format	Scientific Literature
6.	ALA4775288	B	Inhibition of NLRP3 inflammasome activation in PMA-stimulated human THP-1 cells assessed as reduction in cleaved p20 subunit of active caspase-1 secretion at 15 to 45 uM pre-treated for 24 hrs followed by stimulation with LPS for 3 hrs and ATP for 1 hr by Western blot analysis	Homo sapiens	1		tissue-based format	Scientific Literature
7.	ALA4775289	B	Inhibition of NLRP3 inflammasome activation in PMA-stimulated human THP-1 cells assessed as reduction in cleaved p20 subunit of active caspase-1 secretion at 15 to 15 uM pre-treated for 24 hrs followed by stimulation with LPS for 3 hrs and ATP for 1 hr by ELISA analysis	Homo sapiens	1		tissue-based format	Scientific Literature
8.	ALA4775290	B	Inhibition of NLRP3 inflammasome activation in PMA-stimulated human THP-1 cells assessed as reduction in mature IL-1beta secretion at 5 to 15 uM pre-treated for 24 hrs followed by stimulation with LPS for 3 hrs and ATP for 1 hr by ELISA analysis	Homo sapiens	1		tissue-based format	Scientific Literature
9.	ALA4775291	B	Inhibition of NLRP3 inflammasome activation in PMA-stimulated human THP-1 cells assessed as reduction in IL-1beta secretion at 5 to 15 uM pre-treated for 24 hrs followed by stimulation with LPS for 3 hrs and nigericin for 1 hr by ELISA analysis	Homo sapiens	1		tissue-based format	Scientific Literature
10.	ALA4775292	B	Inhibition of NLRP3 inflammasome activation in PMA-stimulated mouse BMDM cells assessed as reduction in IL-1beta secretion at 5 to 15 uM pre-treated for 24 hrs followed by stimulation with LPS for 3 hrs and ATP for 1 hr by ELISA analysis	Mus musculus	1		tissue-based format	Scientific Literature
11.	ALA4775293	F	Inhibition of NLRP3 protein expression in PMA-stimulated human THP-1 cells at 15 to 45 uM pre-treated for 24 hrs followed by stimulation with LPS for 3 hrs by Western blot analysis	Homo sapiens	1		tissue-based format	Scientific Literature
12.	ALA4775294	F	Inhibition of pro-IL-1beta protein expression in PMA-stimulated human THP-1 cells at 15 to 45 uM pre-treated for 24 hrs followed by stimulation with LPS for 3 hrs by Western blot analysis	Homo sapiens	1		tissue-based format	Scientific Literature
13.	ALA4775295	B	Inhibition of NF-kB phosphorylation in PMA-stimulated human THP-1 cells at 15 to 45 uM pre-treated for 24 hrs followed by stimulation with LPS for 4 hrs by Western blot analysis	Homo sapiens	1		tissue-based format	Scientific Literature
14.	ALA4775296	F	Inhibition of Ikappa-Balpha protein expression in PMA-stimulated human THP-1 cells at 15 to 45 uM pre-treated for 24 hrs followed by stimulation with LPS for 4 hrs by Western blot analysis	Homo sapiens	1		tissue-based format	Scientific Literature
15.	ALA4775297	F	Inhibition of IL-1beta mRNA mRNA expression in PMA-stimulated human THP-1 cells at 15 to 45 uM pre-treated for 24 hrs followed by stimulation with LPS for 4 hrs by RT-PCR analysis	Homo sapiens	1		tissue-based format	Scientific Literature
16.	ALA4775298	F	Inhibition of NLRP3 mRNA mRNA expression in PMA-stimulated human THP-1 cells at 15 to 45 uM pre-treated for 24 hrs followed by stimulation with LPS for 4 hrs by RT-PCR analysis	Homo sapiens	1		tissue-based format	Scientific Literature
17.	ALA4775299	F	Inhibition of caspase-1 mRNA mRNA expression in PMA-stimulated human THP-1 cells at 15 to 45 uM pre-treated for 24 hrs followed by stimulation with LPS for 4 hrs by RT-PCR analysis	Homo sapiens	1		tissue-based format	Scientific Literature
18.	ALA4775300	F	Inhibition of TNF-alpha production in PMA-stimulated human THP-1 cells at 45 uM pre-treated for 24 hrs followed by stimulation with LPS for 3 hrs and ATP for 1 hr by ELISA analysis	Homo sapiens	1		tissue-based format	Scientific Literature
19.	ALA4775301	F	Inhibition of TNF-alpha production in PMA-stimulated human THP-1 cells at 5 to 30 uM pre-treated for 24 hrs followed by stimulation with LPS for 3 hrs and ATP for 1 hr by ELISA analysis	Homo sapiens	1		tissue-based format	Scientific Literature
20.	ALA4775302	B	Inhibition of human recombinant NLRP3 ATPase activity assessed as reduction in ATP hydrolysis at 5 to 45 uM incubated for 30 mins before ATP addition and measured after 1 hr by was by luminescent kinase assay	Homo sapiens	1		single protein format	Scientific Literature