# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4714360 | Binding | Inhibition of tyrosinase in mouse B16F10 cells assessed melanin level at 8 uM after 24 hrs relative to control | Mus musculus | 2 | ALA4706702 | cell-based format | Scientific Literature | |
2. | ALA4714362 | Binding | Inhibition of mushroom tyrosinase using L-DOPA as substrate at 10 uM preincubated for 20 mins followed by substrate addition and measured after 10 mins relative to control | Agaricus bisporus | 15 | ALA4706702 | assay format | Scientific Literature | |
3. | ALA4714363 | Binding | Inhibition of mushroom tyrosinase using L-DOPA as substrate at 0.5 uM preincubated for 20 mins followed by substrate addition and measured after 10 mins relative to control | Agaricus bisporus | 15 | ALA4706702 | assay format | Scientific Literature | |
4. | ALA4714364 | Binding | Inhibition of mushroom tyrosinase using L-DOPA as substrate preincubated for 20 mins followed by substrate addition and measured after 10 mins | Agaricus bisporus | 16 | ALA4706702 | assay format | Scientific Literature | |
5. | ALA4714365 | Functional | Antioxidant activity assessed as inhibition of DPPH radical activity at 100 uM incubated for 20 mins by spectrophotometric method relative to control | 16 | ALA4706702 | assay format | Scientific Literature | ||
6. | ALA4714366 | Binding | Uncompetitive inhibition of mushroom tyrosinase assessed as Km at 0.5 uM using L-DOPA as substrate preincubated for 10 mins followed by substrate addition and measured after 10 mins by Lineweaver-Burk plot analysis (Rvb = 37.31 uM) | Agaricus bisporus | 1 | ALA4706702 | assay format | Scientific Literature | |
7. | ALA4714367 | Binding | Uncompetitive inhibition of mushroom tyrosinase assessed as Km at 2.5 uM using L-DOPA as substrate preincubated for 10 mins followed by substrate addition and measured after 10 mins by Lineweaver-Burk plot analysis (Rvb = 37.31 uM) | Agaricus bisporus | 1 | ALA4706702 | assay format | Scientific Literature | |
8. | ALA4714368 | Binding | Uncompetitive inhibition of mushroom tyrosinase assessed as Km at 10 uM using L-DOPA as substrate preincubated for 10 mins followed by substrate addition and measured after 10 mins by Lineweaver-Burk plot analysis (Rvb = 37.31 uM) | Agaricus bisporus | 1 | ALA4706702 | assay format | Scientific Literature | |
9. | ALA4714369 | Binding | Uncompetitive inhibition of mushroom tyrosinase assessed as Vmax at 0.5 uM using L-DOPA as substrate preincubated for 10 mins followed by substrate addition and measured after 10 mins by Lineweaver-Burk plot analysis (Rvb = 8.92 OD/min) | Agaricus bisporus | 1 | ALA4706702 | assay format | Scientific Literature | |
10. | ALA4714370 | Binding | Uncompetitive inhibition of mushroom tyrosinase assessed as Vmax at 2.5 uM using L-DOPA as substrate preincubated for 10 mins followed by substrate addition and measured after 10 mins by Lineweaver-Burk plot analysis (Rvb = 8.92 OD/min) | Agaricus bisporus | 1 | ALA4706702 | assay format | Scientific Literature | |
11. | ALA4714371 | Binding | Uncompetitive inhibition of mushroom tyrosinase assessed as Vmax at 10 uM using L-DOPA as substrate preincubated for 10 mins followed by substrate addition and measured after 10 mins by Lineweaver-Burk plot analysis (Rvb = 8.92 OD/min) | Agaricus bisporus | 1 | ALA4706702 | assay format | Scientific Literature | |
12. | ALA4714372 | Binding | Inhibition of tyrosinase in mouse B16F10 cells assessed reduction in melanin level at 2 uM after 24 hrs | Mus musculus | 2 | ALA4706702 | cell-based format | Scientific Literature | |
13. | ALA4714373 | ADME | Cytotoxicity against mouse B16F10 cells assessed as reduction in cell viability at 2 to 4 uM after 48 hrs by MTT assay | Mus musculus | 2 | ALA4706702 | cell-based format | Scientific Literature | |
14. | ALA4714374 | ADME | Cytotoxicity against mouse B16F10 cells assessed as reduction in cell viability at 8 uM after 48 hrs by MTT assay | Mus musculus | 2 | ALA4706702 | cell-based format | Scientific Literature |