Binding affinity to CLK4 (unknown origin) assessed as change in melting temperature at 10 uM incubated for 10 mins using SYPRO orange dye by RT-PCR-based fluorescence assay
Binding affinity to CLK1 (H148 to I484 residues) (unknown origin) assessed as change in melting temperature at 10 uM incubated for 10 mins using SYPRO orange dye by RT-PCR-based fluorescence assay
Binding affinity to CLK2 (unknown origin) assessed as change in melting temperature at 10 uM incubated for 10 mins using SYPRO orange dye by RT-PCR-based fluorescence assay
Selective index, difference between deltaTm for recombinant wild type CLK1 (H148 to I484 residues) (unknown origin) expressed in Escherichia coli to deltaTm for recombinant CLK1 V324A mutant (unknown origin) expressed in Escherichia coli
Selective index, difference between deltaTm for CLK3 A319V mutant (unknown origin) expressed in Escherichia coli to deltaTm for recombinant wild type CLK3 (R134 to T484 residues) (unknown origin) expressed in Escherichia coli
Binding affinity to CLK3 (R134 to T484 residues) (unknown origin) assessed as change in melting temperature at 10 uM incubated for 10 mins using SYPRO orange dye by RT-PCR-based fluorescence assay