# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4679830 | B | Inhibition of non-phosphorylated CDK4/Cyclin D3 (unknown origin) assessed as reduction in production of ADP using 5-FAM-RRRFRPASPLRGPPK peptide as substrate preincubated with enzyme for 12 mins and measured after 35 mins in presence of ATP by fluorescence-based microfluidic mobility shift assay | Homo sapiens | 5 | protein complex format | Scientific Literature | ||
2. | ALA4679831 | B | Inhibition of non-phosphorylated CDK6/Cyclin D1 (unknown origin) assessed as reduction in production of ADP using 5-FAM-RRRFRPASPLRGPPK peptide as substrate preincubated with enzyme for 12 mins and measured after 35 mins in presence of ATP by fluorescence-based microfluidic mobility shift assay | Homo sapiens | 14 | protein complex format | Scientific Literature | ||
3. | ALA4679832 | B | Inhibition of recombinant full length wild-type phosphorylated CDK2/Cyclin E1 (unknown origin) expressed in baculovirus expression system assessed as reduction in production of ADP using 5-FAM-QSPKKG-CONH2 peptide as substrate preincubated with enzyme for 15 mins followed by further incubation with ATP for 45 mins by fluorescence-based microfluidic mobility shift assay | Homo sapiens | 14 | assay format | Scientific Literature | ||
4. | ALA5236936 | B | Inhibition of CDK2/cyclin E1 (unknown origin) assessed as reduction in substrate peptide phosphorylation using DYRKtide peptide as substrate preincubated for 12 mins followed by ATP addition and measured for 45 mins by mobility shift assay | Homo sapiens | 22 | protein complex format | Scientific Literature | ||
5. | ALA5236937 | B | Inhibition of CDK4/cyclin D1 (unknown origin) assessed as reduction in substrate peptide phosphorylation using DYRKtide peptide as substrate preincubated for 12 mins followed by ATP addition and measured for 45 mins by mobility shift assay | Homo sapiens | 22 | protein complex format | Scientific Literature | ||
6. | ALA5236938 | B | Inhibition of CDK6/cyclin D1 (unknown origin) assessed as reduction in substrate peptide phosphorylation using DYRKtide peptide as substrate preincubated for 12 mins followed by ATP addition and measured for 45 mins by mobility shift assay | Homo sapiens | 22 | protein complex format | Scientific Literature | ||
7. | ALA5236939 | B | Inhibition of CDK1/Cyclin-A2 (unknown origin) assessed as reduction in substrate peptide phosphorylation using DYRKtide peptide as substrate preincubated for 12 mins followed by ATP addition and measured for 45 mins by mobility shift assay | Homo sapiens | 22 | assay format | Scientific Literature | ||
8. | ALA5236940 | B | Inhibition of CDK9/Cyclin-T1 (unknown origin) assessed as reduction in substrate peptide phosphorylation using DYRKtide peptide as substrate preincubated for 12 mins followed by ATP addition and measured for 45 mins by mobility shift assay | Homo sapiens | 22 | protein complex format | Scientific Literature | ||
9. | ALA5236941 | B | Selectivity ratio, ratio of Ki for inhibition of CDK1/Cyclin-A2 (unknown origin) to Ki for inhibition of CDK2/cyclin E1 (unknown origin) | Homo sapiens | 19 | assay format | Scientific Literature | ||
10. | ALA5236942 | A | Metabolic stability in human liver microsomes assessed as intrinsic clearance per mg of protein | Homo sapiens | 20 | microsome format | Scientific Literature |