# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4715224 | B | Inhibition of human His-tagged LIN28A (16 to 187 residues) interaction with mouse preE-let-7f-Cy3 miRNA pretreated with protein for 2 hrs followed by incubation with miRNA and measured after 15 mins by EMSA method | 1 | ALA4706754 | assay format | Scientific Literature | ||
2. | ALA4715225 | B | Inhibition of human His-tagged LIN28A (16 to 187 residues) interaction with FAM-labelled preE-let-7f-1 miRNA (unknown origin) pretreated with protein for 30 mins followed by miRNA addition and measured after 15 mins by fluorescence polarization assay | Homo sapiens | 31 | ALA4706754 | assay format | Scientific Literature | |
3. | ALA4715226 | B | Inhibition of LIN28A cold shock domain (16 to 126 residues) (unknown origin) assessed as increase in thermal stability measured after 45 mins by differential scanning fluorimetry | Homo sapiens | 1 | ALA4706754 | single protein format | Scientific Literature | |
4. | ALA4715227 | B | Inhibition of LIN28/let-7 miRNA interaction in human JAR cells assessed as increase in cellular let-7a mature miRNA level at 5 to 20 uM measured after 24 hrs by RT-qPCR analysis | Homo sapiens | 1 | ALA4706754 | cell-based format | Scientific Literature | |
5. | ALA4715228 | B | Inhibition of LIN28/let-7 miRNA interaction in human JAR cells assessed as increase in cellular let-7g mature miRNA level at 5 to 20 uM measured after 24 hrs by RT-qPCR analysis | Homo sapiens | 1 | ALA4706754 | cell-based format | Scientific Literature | |
6. | ALA4715234 | B | Stabilization of interaction of GST-fused C-terminal region of PMA2 YDI (unknown origin)/eGFP-fused tobacco 14-3-3 expressed in Escherichia coli incubated for 2 hrs by fluorescence based analysis relative to PPI-1 | 2 | ALA4706754 | assay format | Scientific Literature |