Hepatotoxicity in primary human hepatocytes assessed as increase in lactate dehydrogenase release at 500 to 1000 uM measured after 3 to 12 hrs by fluorescence based analysis
Hepatotoxicity in primary human hepatocytes assessed as decrease in GSH level at 500 to 1000 uM measured after 3 to 12 hrs by Thiol tracker violet dye based fluorescence analysis
Hepatotoxicity in human HepaRG cells assessed as increase in lactate dehydrogenase release at 50 to 5000 uM measured after 6 to 24 hrs by fluorescence based analysis
Hepatotoxicity in human HepaRG cells assessed as decrease in GSH level at 50 to 5000 uM measured after 6 to 24 hrs by Thiol tracker violet dye based fluorescence analysis
Hepatotoxicity in CD1 mouse assessed as loss of chicken wire hepatic tight junctions at 600 mg/kg, po measured after 12 hrs by ZO-1 staining based optical microscopy
Hepatotoxicity in CD1 mouse assessed as liver architecture at 600 mg/kg, po measured after 12 hrs by hematoxylin and eosin staining based optical microscopy
Inhibition of CYP3A4 (unknown origin) in baculosomes preincubated for 20 mins followed by addition of CYP enzyme-specific substrate and NADP+ and measured after 30 mins by fluorescence based analysis relative to control
Inhibition of CYP2E1 (unknown origin) in baculosomes preincubated for 20 mins followed by addition of CYP enzyme-specific substrate and NADP+ and measured after 30 mins by fluorescence based analysis relative to control
Inhibition of CYP3A4 (unknown origin) in baculosomes preincubated for 20 mins followed by addition of CYP enzyme-specific substrate and NADP+ and measured after 30 mins by fluorescence based analysis
Inhibition of CYP2D6 (unknown origin) in baculosomes preincubated for 20 mins followed by addition of CYP enzyme-specific substrate and NADP+ and measured after 30 mins by fluorescence based analysis
Analgesic activity in po dosed CD1 mouse assessed as inhibition of acetic acid-induced abdominal writhing treated with acetic acid 25 mins post drug administration and measured after 5 mins for 10 mins
Analgesic activity in ip dosed complete freund's adjuvant-induced Sprague Dawley rat model of hyperalgesia assessed as inhibition of inflammatory pain measured after 30 mins by von Frey analgesimetric method