| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA4670358 | F | Inverse agonist activity at SNAP-tagged human GHSR expressed in HEK293 cells assessed as inhibition of ghrelin-induced IP1 accumulation at 10^-5 M measured after 30 mins by IP1-d2 derivative based HTRF assay relative to ghrelin | Homo sapiens | 16 | cell-based format | Scientific Literature | ||
| 2. | ALA4670371 | F | Inverse agonist activity at SNAP-tagged human GHSR expressed in HEK293 cells assessed as inhibition of ghrelin-induced IP1 accumulation at 10^-5 M measured after 30 mins by HTRF assay relative to control | Homo sapiens | 23 | cell-based format | Scientific Literature | ||
| 3. | ALA4670377 | B | Inverse agonist activity at wild type human GHSR assembled into lipid nanodiscs assessed as reduction in ghrelin-induced decrease in proximity ratio at 10 uM after 30 mins by fluorophore-labelled TM1/TM6 probe-based FRET assay | Homo sapiens | 4 | single protein format | Scientific Literature | ||
| 4. | ALA4670378 | F | Inverse agonist activity at wild type human GHSR assessed as inhibition of ghrelin-induced BODIPY-FL GTPgammaS binding to Gq protein at 10 uM preincubated for 30 mins in presence of Galphaq and Gbeta1gamma2 followed by BODIPY-FL GTPgammaS addition and measured after 10 mins by lipid nanodisc-based fluorescence spectrophotometric method | Homo sapiens | 4 | assay format | Scientific Literature | ||
| 5. | ALA4670379 | F | Inverse agonist activity at SNAP-tagged human GHSR expressed in HEK293 cells assessed as inhibition of ghrelin-induced IP1 response measured after 30 mins by HTRF assay | Homo sapiens | 23 | cell-based format | Scientific Literature | ||
| 6. | ALA4670380 | F | Inverse agonist activity at SNAP-tagged human GHSR expressed in HEK293 cells assessed as inhibition of ghrelin-induced IP1 accumulation at 10^-5 M measured after 30 mins by IP1-d2 derivative based HTRF assay relative to control | Homo sapiens | 16 | cell-based format | Scientific Literature | ||
| 7. | ALA4670384 | B | Displacement of red-ghrelin from SNAP-tagged human GHSR expressed in HEK293 cells incubated for 3 hrs at 4 degC or for 1 hr at room temperature by HTRF assay | Homo sapiens | 46 | cell-based format | Scientific Literature |
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