# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4772283 | Binding | Displacement of [125I]NPFF from human NPFFR1 expressed in CHO cell membranes incubated for 120 mins by scintillation/luminescence counting method | Homo sapiens | 2 | ALA4765381 | cell membrane format | Scientific Literature | |
2. | ALA4772284 | Functional | Antagonist activity at human NPFFR1 expressed in CHO-RD-HGA16 cells assessed as reduction in NPFF-induced calcium influx by measuring apparent dissociation constant Ke preincubated for 15 mins followed by NPFF addition by calcium-5 dye based fluorescence method | Homo sapiens | 2 | ALA4765381 | cell-based format | Scientific Literature | |
3. | ALA4772285 | Functional | Antagonist activity at human NPFFR1 expressed in CHO-K1 cells assessed as apparent dissociation constant Ke by Eu-cAMP tracer based TR-FRET assay | Homo sapiens | 2 | ALA4765381 | cell-based format | Scientific Literature | |
4. | ALA4772286 | Binding | Displacement of [125I]NPFF from human NPFFR2 expressed in CHO cell membranes incubated for 120 mins by scintillation/luminescence counting method | Homo sapiens | 2 | ALA4765381 | cell membrane format | Scientific Literature | |
5. | ALA4772287 | Functional | Antagonist activity at human NPFFR2 expressed in CHO-RD-HGA16 cells assessed as reduction in NPFF-induced calcium influx by measuring apparent dissociation constant Ke preincubated for 15 mins followed by NPFF addition by calcium-5 dye based fluorescence method | Homo sapiens | 2 | ALA4765381 | cell-based format | Scientific Literature | |
6. | ALA4772294 | Functional | Antagonist activity at human NPFFR2 expressed in CHO-K1 cells assessed as apparent dissociation constant Ke by Eu-cAMP tracer based TR-FRET assay | Homo sapiens | 2 | ALA4765381 | cell-based format | Scientific Literature |