#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA4831563	Toxicity	Acute toxicity in BALB/c nude mouse assessed as heart damage at 200 mg/kg, po administered twice daily for 4 weeks by H and E staining based optical microscopic analysis	Mus musculus	1	ALA4831477	organism-based format	Scientific Literature
2.	ALA4831564	Toxicity	Acute toxicity in BALB/c nude mouse assessed as liver damage at 200 mg/kg, po administered twice daily for 4 weeks by H and E staining based optical microscopic analysis	Mus musculus	1	ALA4831477	organism-based format	Scientific Literature
3.	ALA4831565	Binding	Inhibition of recombinant human GLS1 expressed in Escherichia coli strain BL21 (DE3) preincubated for 10 mins before glutamine addition and measured after 60 mins by bovine liver glutamate dehydrogenase based coupled enzyme assay	Homo sapiens	20	ALA4831477	assay format	Scientific Literature
4.	ALA4831566	Binding	Binding affinity to human GLS1 expressed in Escherichia coli strain BL21 (DE3) assessed as change in melting temperature by SYPRO orange dye based protein thermal shift assay	Homo sapiens	20	ALA4831477	assay format	Scientific Literature
5.	ALA4831567	Functional	Antiproliferative activity against human HCT116 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	Homo sapiens	20	ALA4831477	cell-based format	Scientific Literature
6.	ALA4831568	Functional	Antiproliferative activity against human MDA-MB-436 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	Homo sapiens	20	ALA4831477	cell-based format	Scientific Literature
7.	ALA4831569	Binding	Noncompetitive inhibition of human GLS1 expressed in Escherichia coli strain BL21 (DE3) assessed as decrease in Vmax using varying concentrations of glutamine as substrate by Michaelis-Menten plot analysis	Homo sapiens	1	ALA4831477	assay format	Scientific Literature
8.	ALA4831570	Binding	Binding affinity to human GLS1 expressed in Escherichia coli strain BL21 (DE3) assessed as change in melting temperature at ratio of 1:2 by SYPRO orange dye based protein thermal shift assay	Homo sapiens	1	ALA4831477	assay format	Scientific Literature
9.	ALA4831571	Binding	Binding affinity to human GLS1 expressed in Escherichia coli strain BL21 (DE3) assessed as change in melting temperature at ratio of 1:5 by SYPRO orange dye based protein thermal shift assay	Homo sapiens	1	ALA4831477	assay format	Scientific Literature
10.	ALA4831572	Binding	Binding affinity to human GLS1 expressed in Escherichia coli strain BL21 (DE3) assessed as dissociation constant by surface plasmon resonance analysis	Homo sapiens	1	ALA4831477	assay format	Scientific Literature
11.	ALA4831573	Binding	Binding affinity to human GLS1 expressed in Escherichia coli strain BL21 (DE3) assessed as association rate constant by surface plasmon resonance analysis	Homo sapiens	1	ALA4831477	assay format	Scientific Literature
12.	ALA4831574	Binding	Binding affinity to human GLS1 expressed in Escherichia coli strain BL21 (DE3) assessed as dissociation rate constant by surface plasmon resonance analysis	Homo sapiens	1	ALA4831477	assay format	Scientific Literature
13.	ALA4831575	Binding	Binding affinity to human GLS1 expressed in Escherichia coli strain BL21 (DE3) by ITC analysis	Homo sapiens	2	ALA4831477	assay format	Scientific Literature
14.	ALA4831577	Functional	Inhibition of clone formation in human HCT-116 cells at 0.1 to 2 microM incubated for 14 days by crystal violet staining based assay	Homo sapiens	1	ALA4831477	cell-based format	Scientific Literature
15.	ALA4831578	Binding	Inhibition of GLS1 in human HCT-116 cells assessed as accumulation of glutamine level at 50 to 200 nM by LC-MS/MS analysis	Homo sapiens	1	ALA4831477	cell-based format	Scientific Literature
16.	ALA4831579	Binding	Inhibition of GLS1 in human HCT-116 cells assessed as reduction in glutamate level at 50 to 200 nM by LC-MS/MS analysis	Homo sapiens	1	ALA4831477	cell-based format	Scientific Literature
17.	ALA4831580	Binding	Inhibition of GLS1 in human HCT-116 cells assessed as reduction in aspartate level at 50 to 200 nM after 12 hrs by LC-MS/MS analysis	Homo sapiens	1	ALA4831477	cell-based format	Scientific Literature
18.	ALA4831581	Binding	Inhibition of GLS1 in human HCT-116 cells assessed as reduction in glutathione level at 50 to 200 nM after 12 hrs by LC-MS/MS analysis	Homo sapiens	1	ALA4831477	cell-based format	Scientific Literature
19.	ALA4831582	Binding	Inhibition of GLS1 in human HCT-116 cells assessed as reduction in fumarate level at 50 to 200 nM after 12 hrs by LC-MS/MS analysis	Homo sapiens	1	ALA4831477	cell-based format	Scientific Literature
20.	ALA4831583	Binding	Inhibition of GLS1 in human HCT-116 cells assessed as reduction in malate level at 50 to 200 nM after 12 hrs by LC-MS/MS analysis	Homo sapiens	1	ALA4831477	cell-based format	Scientific Literature