Antiviral activity against PEDV 85-7 infected in Marc145 cells assessed as inhibition of viral replication by measuring reduction in mRNA level at 10 uM after 24 hrs by qPCR analysis
Antiviral activity against PEDV 85-7 infected in Marc145 cells assessed as reduction in PDEV protein level at 10 uM after 24 hrs by indirect immunofluorescence assay
Antiviral activity against PEDV 85-7 infected in Marc145 cells assessed as down regulation of Hsc70 mRNA expression at 10 uM after 24 hrs by qPCR analysis
Antiviral activity against PEDV 85-7 infected in Marc145 cells assessed as down regulation of Hsc70 protein expression after 24 hrs by Western blotting analysis
Antiviral activity against PEDV 85-7 infected in Marc145 cells expressing pEGFP-N1/Hsc70 protein assessed as decrease in viral RNA level at 20 uM after 24 hrs by qPCR analysis
Antiviral activity against PEDV 85-7 infected in Marc145 cells expressing pEGFP-N1/Hsc70 protein assessed as decrease in PEDV-N-protein level at 20 uM after 24 hrs by qPCR analysis
Suppression of Hsc70 expression in African green monkey Marc145 cells assessed as Hsc70 mRNA destabilization by measuring half life at 20 uM (Rvb = 6.51 hrs)
Antiviral activity against PEDV (NK-2) inoculated in piglets assessed as reduction in multifocal diffusion of villous atrophy at 50 mg/kg, po administered every 24 hrs for 72 hrs by HE staining based microscopic analysis
Antiviral activity against PEDV (NK-2) inoculated in piglets assessed as reduction in swelling sloughing and attenuation of villi at 50 mg/kg, po administered every 24 hrs for 72 hrs by HE staining based microscopic analysis
Antiviral activity against PEDV (NK-2) inoculated in piglets assessed as reduction in fusion of numerous villi at 50 mg/kg, po administered every 24 hrs for 72 hrs by HE staining based microscopic analysis
Antiviral activity against PEDV (NK-2) inoculated in piglets assessed as reduction in viral replication in intestine at 50 mg/kg, po administered every 24 hrs for 72 hrs by immunofluorescence assay