# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4822200 | Binding | Inhibition of recombinant human GST-tagged TET2 (1099 to 1936 residues) expressed in Escherichia coli BL21 (DE3)pLysS assessed as reduction in 5hmc level incubated for 2 hrs by ELISA | Homo sapiens | 8 | ALA4819102 | assay format | Scientific Literature | |
2. | ALA4822201 | Binding | Potency index, ratio of IC50 of NOG to IC50 of compound for inhibition of recombinant human GST-tagged TET2 (1099 to 1936 residues) expressed in Escherichia coli BL21 (DE3)pLysS assessed as reduction in 5hmc level incubated for 2 hrs by ELISA | Homo sapiens | 3 | ALA4819102 | assay format | Scientific Literature | |
3. | ALA4822202 | Binding | Inhibition of recombinant human N-terminal Flag-tagged TET1 (1418 to 2136 residues) expressed in Baculovirus infected Sf9 cells assessed as reduction in 5hmc level incubated for 2 hrs by ELISA | Homo sapiens | 1 | ALA4819102 | cell-based format | Scientific Literature | |
4. | ALA4822203 | Binding | Inhibition of recombinant human N-terminal Flag-tagged C-terminal His-tagged TET3 (824 to 1795 residues) expressed in Baculovirus infected Sf9 cells assessed as reduction in 5hmc level incubated for 2 hrs by ELISA | Homo sapiens | 1 | ALA4819102 | cell-based format | Scientific Literature | |
5. | ALA4822204 | Binding | Binding affinity to human recombinant TET2 (1099 to 1936) catalytic domain assessed as dissociation constant by micro-scale thermophoresis analysis | Homo sapiens | 4 | ALA4819102 | single protein format | Scientific Literature | |
6. | ALA4822205 | Binding | Reversible inhibition of recombinant human GST-tagged TET2 (1099 to 1936 residues) expressed in Escherichia coli BL21 (DE3)pLysS assessed as restoration of enzyme activity in presence of alpha-KG at 20 uM incubated for 2 hrs by ELISA | Homo sapiens | 1 | ALA4819102 | assay format | Scientific Literature |