#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA4820536	F	Anti-osteoporosis activity in C57BL/6 mouse BMM cells assessed as inhibition of M-CSF/RANKL-induced osteoclastogenesis by measuring reduction in multinucleated TRAP+ cells incubated for 5 days with media replenishment for every 48 hrs measured by TRAP-staining based analysis	Mus musculus	37		cell-based format	Scientific Literature
2.	ALA4820538	A	Cytotoxicity against C57BL/6 mouse BMM cells assessed as reduction in cell viability at 30 uM measured after 48 hrs in presence of M-CSF by CCK8 assay	Mus musculus	1		cell-based format	Scientific Literature
3.	ALA4820539	F	Anti-osteoporosis activity in C57BL/6 mouse BMM cells assessed as inhibition of M-CSF/RANKL-induced osteoclastogenesis by measuring reduction in size and number of osteoclasts incubated for 5 days with media replenishment for every 48 hrs measured by TRAP-staining based analysis	Mus musculus	1		cell-based format	Scientific Literature
4.	ALA4820540	F	Anti-osteoporosis activity in C57BL/6 mouse BMM cells assessed as inhibition of M-CSF/RANKL-induced bone resorption by measuring suppression of reabsorbing pits formation after 48 hrs by hydroxyapatite assay using Leica inverted microscope	Mus musculus	2		cell-based format	Scientific Literature
5.	ALA4820541	F	Anti-osteoporosis activity in C57BL/6 mouse BMM cells assessed as inhibition of M-CSF/RANKL-induced bone resorption at 10 uM after 48 hrs by hydroxyapatite assay using Leica inverted microscope	Mus musculus	1		cell-based format	Scientific Literature
6.	ALA4820542	F	Downregulation of osteoclastogenesis-associated gene expression in C57BL/6 mouse BMM cells assessed as inhibition of M-CSF/RANKL-induced TRAcP expression at 5 uM after 5 days by qRT-PCR analysis	Mus musculus	1		cell-based format	Scientific Literature
7.	ALA4820543	F	Downregulation of osteoclastogenesis-associated gene expression in C57BL/6 mouse BMM cells assessed as inhibition of M-CSF/RANKL-induced CTSK expression at 5 uM after 5 days by qRT-PCR analysis	Mus musculus	1		cell-based format	Scientific Literature
8.	ALA4820544	F	Downregulation of osteoclastogenesis-associated gene expression in C57BL/6 mouse BMM cells assessed as inhibition of M-CSF/RANKL-induced NFATc1 expression at 5 uM after 5 days by qRT-PCR analysis	Mus musculus	1		cell-based format	Scientific Literature
9.	ALA4820545	F	Downregulation of osteoclastogenesis-associated gene expression in C57BL/6 mouse BMM cells assessed as inhibition of M-CSF/RANKL-induced MMP9 expression at 5 uM after 5 days by qRT-PCR analysis	Mus musculus	1		cell-based format	Scientific Literature
10.	ALA4820546	B	Inhibition of RANKL-induced NFkappaB signaling pathway in mouse RAW264.7 cells assessed as reduction in NFkappaB p65 phosphorylation preincubated for 4 hrs followed by RANKL stimulation for 10 mins and measured by Western blot analysis	Mus musculus	1		cell-based format	Scientific Literature
11.	ALA4820547	F	Effect on NFkappaB p65 expression in RANKL-stimulated mouse RAW264.7 cells at 1 to 10 uM preincubated for 4 hrs followed by RANKL stimulation for 10 mins and measured by Western blot analysis	Mus musculus	1		tissue-based format	Scientific Literature
12.	ALA4820548	B	Inhibition of RANKL-induced NFkappaB signaling pathway in mouse RAW264.7 cells assessed as reduction in IkappaB alpha phosphorylation at 10 uM preincubated for 4 hrs followed by RANKL stimulation for 10 mins and measured by Western blot analysis	Mus musculus	1		cell-based format	Scientific Literature
13.	ALA4820549	B	Inhibition of RANKL-induced NFkappaB signaling pathway in mouse RAW264.7 cells assessed as reduction in IkappaB alpha degradation at 10 uM preincubated for 4 hrs followed by RANKL stimulation for 10 mins and measured by Western blot analysis	Mus musculus	1		cell-based format	Scientific Literature
14.	ALA4820550	B	Inhibition of RANKL-induced NFkappaB signaling pathway in mouse RAW264.7 cells assessed as reduction in NFkappaB p65 nuclear translocation at 10 uM preincubated for 4 hrs followed by RANKL stimulation for 10 mins and measured by DAPI staining based immunofluorescence analysis	Mus musculus	1		cell-based format	Scientific Literature
15.	ALA4820551	F	Induction of osteoblast differentiation in mouse C3H10T1 /2 cells assessed as increase in ALP expression at 1 to 10 uM measured after 7 days by ALP staining based assay	Mus musculus	1		cell-based format	Scientific Literature
16.	ALA4820552	F	Induction of osteoblast differentiation in mouse C3H10T1/2 cells assessed as increase in calcium nodule formation at 1 to 10 uM measured after 14 days by alizarin red staining assay	Mus musculus	1		cell-based format	Scientific Literature
17.	ALA4820553	F	Antiosteoporotic activity against OVX-induced bone loss C57BL/6 mouse model assessed as increase in trabecular bone mass in femoral metaphysis at 10 mg/kg, po QD for 4 weeks by microCT analysis	Mus musculus	1		organism-based format	Scientific Literature
18.	ALA4820554	F	Antiosteoporotic activity against OVX-induced bone loss C57BL/6 mouse model assessed as increase in bone volume/total bone volume in femoral metaphysis at 10 mg/kg, po QD for 4 weeks	Mus musculus	2		organism-based format	Scientific Literature
19.	ALA4820555	F	Antiosteoporotic activity against OVX-induced bone loss C57BL/6 mouse model assessed as increase in trabecular thickness in femoral metaphysis at 10 mg/kg, po QD for 4 weeks	Mus musculus	2		organism-based format	Scientific Literature
20.	ALA4820556	F	Antiosteoporotic activity against OVX-induced bone loss C57BL/6 mouse model assessed as increase in trabecular number in femoral metaphysis at 10 mg/kg, po QD for 4 weeks	Mus musculus	2		organism-based format	Scientific Literature