# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4810314 | Binding | Inhibition of SARS-CoV-2 3CLpro preincubated for 30 mins followed by addition of (Dabcyl)KTSAVLQSGFRKM(Glu) peptide substrate and measured after 1.5 hrs by FRET assay | Severe acute respiratory syndrome coronavirus 2 | 15 | ALA4808212 | single protein format | Scientific Literature | |
2. | ALA4810323 | Binding | Binding affinity to SARS coronavirus 1 3CL protease using Dabcyl-KTSAVLQ/SGFRKME-Edans as substrate measured by FRET assay | SARS coronavirus | 1 | ALA4808212 | assay format | Scientific Literature | |
3. | ALA5117299 | Binding | Inhibition of eGFP fused SARS-CoV2 main protease transfected in HEK293T/17 cells incubated for 72 hrs by fluorescence based flow cytometry analysis | Severe acute respiratory syndrome coronavirus 2 | 26 | ALA5113464 | cell-based format | Scientific Literature | |
4. | ALA5117301 | Functional | Antiviral activity against SARS-Cov2 USA-WA1/2020 infected in African green monkey Vero E6 cells assessed as viral plaque reduction measured after 3 days by plaque reduction neutralization test | Severe acute respiratory syndrome coronavirus 2 | 7 | ALA5113464 | organism-based format | Scientific Literature | |
5. | ALA5117306 | Toxicity | Cytotoxicity against HEK293T/17 cells by MTT assay | Homo sapiens | 7 | ALA5113464 | cell-based format | Scientific Literature | |
6. | ALA5117307 | Binding | Inhibition of N-terminal His/SUMO tagged recombinant SARS-CoV2 main protease expressed in Escherichia coli BL21 (DE3) cells using DABCYL-Lys-Thr-Ser-Ala-Val-Leu-Gln-Ser-Gly-Phe-Arg-Lys-MetGlu-EDANS as substrate preincubated with enzyme for 30 mins followed by substrate addition and measured after 30 mins by fluorescence based assay | Severe acute respiratory syndrome coronavirus 2 | 6 | ALA5113464 | cell-based format | Scientific Literature | |
7. | ALA5117308 | Toxicity | Selectivity index, ratio of CC50 for cytotoxicity against HEK293T/17 cells by MTT assay to EC50 for antiviral activity against SARS-Cov2 USA-WA1/2020 infected in African green monkey Vero E6 cells assessed as viral plaque reduction by plaque reduction neutralization test | 4 | ALA5113464 | cell-based format | Scientific Literature | ||
8. | ALA5247336 | Binding | Binding affinity to SARS CoV Main protease incubated for 10 mins by FRET assay | Severe acute respiratory syndrome-related coronavirus | 2 | ALA5244279 | assay format | Scientific Literature | |
9. | ALA5247337 | Functional | Antiviral activity against SARS CoV infected in African green monkey Vero E6 cells measured after 48 hrs by plaque assay | Severe acute respiratory syndrome-related coronavirus | 1 | ALA5244279 | organism-based format | Scientific Literature | |
10. | ALA5247338 | Toxicity | Cytotoxicity against African green monkey Vero E6 cells assessed as reduction of cell viability by CCK8 assay | Chlorocebus sabaeus | 1 | ALA5244279 | cell-based format | Scientific Literature |