| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA4833997 | F | Inhibition of LPS-induced NO production in mouse RAW264.7 cells preincubated for 2 hrs followed by LPS stimulation for 24 hrs by Griess reagent based assay | Mus musculus | 25 | cell-based format | Scientific Literature | ||
| 2. | ALA4833998 | A | Cytotoxicity against mouse RAW264.7 cells assessed as effect on cell viability up to 20 uM measured after 24 hrs by CCK8 assay | Mus musculus | 24 | cell-based format | Scientific Literature | ||
| 3. | ALA4833999 | F | Inhibition of LPS-induced PGE2 production in mouse RAW264.7 cells preincubated for 2 hrs followed by LPS stimulation and measured after 24 hrs by ELISA assay | Mus musculus | 1 | cell-based format | Scientific Literature | ||
| 4. | ALA4834000 | F | Inhibition of LPS-induced iNOS protein expression in mouse RAW264.7 cells at 0.75 to 3 uM preincubated for 2 hrs followed by LPS stimulation for 24 hrs by Western blot analysis | Mus musculus | 1 | cell-based format | Scientific Literature | ||
| 5. | ALA4834001 | F | Inhibition of LPS-induced COX2 protein expression in mouse RAW264.7 cells at 0.75 to 3 uM preincubated for 2 hrs followed by LPS stimulation for 24 hrs by Western blot analysis | Mus musculus | 1 | cell-based format | Scientific Literature | ||
| 6. | ALA4834002 | F | Anti-inflammatory activity against LPS-stimulated mouse RAW264.7 cells assessed as decrease in IL-1 beta production at 0.75 to 3 uM preincubated for 2 hrs followed by LPS stimulation and measured after 24 hrs by ELISA assay | Mus musculus | 1 | cell-based format | Scientific Literature | ||
| 7. | ALA4834003 | F | Anti-inflammatory activity against LPS-stimulated mouse RAW264.7 cells assessed as decrease in IL-6 production at 0.75 to 3 uM preincubated for 2 hrs followed by LPS stimulation and measured after 24 hrs by ELISA assay | Mus musculus | 1 | cell-based format | Scientific Literature | ||
| 8. | ALA4834004 | F | Anti-inflammatory activity against LPS-stimulated mouse RAW264.7 cells assessed as decrease in TNF-alpha production at 0.75 to 3 uM preincubated for 2 hrs followed by LPS stimulation and measured after 24 hrs by ELISA assay | Mus musculus | 1 | cell-based format | Scientific Literature | ||
| 9. | ALA4834008 | B | Inhibition of IKKalpha/beta in LPS-stimulated mouse RAW264.7 cells assessed as decrease in IkappaB degradation at 3 uM preincubated for 2 hrs followed by LPS stimulation for 10 mins by immunoblotting assay | Mus musculus | 1 | tissue-based format | Scientific Literature | ||
| 10. | ALA4834009 | B | Inhibition of IKKalpha/beta in LPS-stimulated mouse RAW264.7 cells assessed as inhibition of NFkB p65 nuclear translocation at 3 uM preincubated for 2 hrs followed by LPS stimulation for 30 mins by immunoblotting assay | Mus musculus | 1 | tissue-based format | Scientific Literature | ||
| 11. | ALA4834010 | B | Inhibition of JNK phosphorylation in LPS-induced mouse RAW264.7 cells at 0.75 to 3 uM preincubated for 2 hrs followed by LPS stimulation and measured after 30 mins by immunoblotting assay | Mus musculus | 1 | tissue-based format | Scientific Literature | ||
| 12. | ALA4834011 | B | Inhibition of ERK phosphorylation in LPS-induced mouse RAW264.7 cells at 0.75 to 3 uM preincubated for 2 hrs followed by LPS stimulation and measured after 30 mins by immunoblotting assay | Mus musculus | 1 | tissue-based format | Scientific Literature | ||
| 13. | ALA4834012 | B | Inhibition of p38 MAPK phosphorylation in LPS-induced mouse RAW264.7 cells at 0.75 to 3 uM preincubated for 2 hrs followed by LPS stimulation and measured after 30 mins by immunoblotting assay | Mus musculus | 1 | tissue-based format | Scientific Literature | ||
| 14. | ALA4834013 | B | Inhibition of IKKalpha/beta in human HEK293T cells assessed as reduction in thermal stability at 50 uM incubated for 30 mins followed by heating at temperature between 58 to 60 degC for 3 mins by CETSA analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 15. | ALA4834014 | B | Inhibition of IKKalpha/beta phosphorylation in LPS-induced mouse RAW264.7 cells incubated for 2 hrs followed by LPS stimulation and measured after 5 mins by immunoblotting assay | Mus musculus | 1 | tissue-based format | Scientific Literature | ||
| 16. | ALA4834015 | F | Inhibition of LPS-induced NO production in mouse RAW264.7 cells at 3 uM preincubated with DTT for 30 mins and later incubated with cells for 2 hrs followed by LPS stimulation for 24 hrs by Griess-reagent based assay | Mus musculus | 1 | cell-based format | Scientific Literature | ||
| 17. | ALA4834016 | B | Inhibition of IKKalpha/beta in LPS-stimulated mouse RAW264.7 cells assessed as inhibition of IkappaB alpha phosphorylation at 3 uM preincubated with DTT for 30 mins and later incubated with cells for 2 hrs followed by LPS stimulation for 5 mins by immunoblot anlaysis | Mus musculus | 1 | tissue-based format | Scientific Literature | ||
| 18. | ALA4834017 | B | Inhibition of IKKalpha/beta in LPS-stimulated mouse RAW264.7 cells assessed as inhibition of IkappaB alpha degradation at 3 uM preincubated with DTT for 30 mins and later incubated with cells for 2 hrs followed by LPS stimulation for 10 mins by immunoblot anlaysis | Mus musculus | 1 | tissue-based format | Scientific Literature | ||
| 19. | ALA4834018 | B | Inhibition of IKKalpha/beta in LPS-stimulated mouse RAW264.7 cells assessed as inhibition of IkappaB alpha phosphorylation preincubated for 2 hrs followed by LPS stimulation for 5 mins by immunoblot anlaysis | Mus musculus | 1 | tissue-based format | Scientific Literature | ||
| 20. | ALA4834019 | B | Covalent binding affinity to dithiothreitol assessed as compound-DTT adduct formation at 50 uM incubated for 30 mins by UPLC-MS analysis | 1 | assay format | Scientific Literature |
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