# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4808299 | B | Displacement of [125I] [NIe75,Tyr77]Pyr-apelin-13 from YFP-tagged human APJ receptor expressed in HEK293 cells incubated for 1 hr by gamma counting analysis | Homo sapiens | 34 | cell-based format | Scientific Literature | ||
2. | ALA4808300 | F | Agonist activity at human APJ receptor expressed in HEK293T cells co-expressing Galphai1 RlucII assessed as dissociation of Galphai1 from Gbetta/gamma subunit measured using coelenterazine luciferase substrate incubated for 5 mins by BRET assay | Homo sapiens | 34 | cell-based format | Scientific Literature | ||
3. | ALA4808301 | F | Agonist activity at human APJ receptor expressed in HEK293T cells co-expressing PDZ-Rho-Gef-RLucll and rGFP-CAAX assessed as induction of recruitment of Galpha12 to cell membrane using coelenterazine luciferase substrate incubated for 5 mins by BRET assay | Homo sapiens | 34 | cell-based format | Scientific Literature | ||
4. | ALA4808302 | F | Agonist activity at human APJ receptor expressed in HEK293 cells co-expressing Rlucll-beta-arrestin2 assessed as induction of beta-arrestin2 recruitment to receptor incubated for 30 mins by BRET assay | Homo sapiens | 34 | cell-based format | Scientific Literature | ||
5. | ALA4808303 | A | Stability in Sprague-Dawley rat plasma assessed as half life measured up to 24 hrs by UPLC-MS analysis | Rattus norvegicus | 15 | cell-free format | Scientific Literature | ||
6. | ALA4808304 | A | Stability of the compound assessed as recombinant rat ACE2-mediated degradation measured up to 24 hrs by UPLC-MS analysis | Rattus norvegicus | 3 | single protein format | Scientific Literature | ||
7. | ALA4808305 | A | AUCtot in Sprague-Dawley rat plasma at 3 mg/kg, iv bolus injection measured up to 240 mins by LC-MS/MS analysis | Rattus norvegicus | 3 | organism-based format | Scientific Literature | ||
8. | ALA4808306 | A | Half life in Sprague-Dawley rat plasma at 3 mg/kg, iv bolus injection measured up to 240 mins by LC-MS/MS analysis | Rattus norvegicus | 3 | organism-based format | Scientific Literature | ||
9. | ALA4808307 | A | Clearance in Sprague-Dawley rat plasma at 3 mg/kg, iv bolus injection measured up to 240 mins by LC-MS/MS analysis | Rattus norvegicus | 3 | organism-based format | Scientific Literature | ||
10. | ALA4808308 | F | Hypotensive effect in Sprague-Dawley rat assessed as drop in mean arterial blood pressure at 19.6 nmol/kg, iv bolus injection measured after 1 min | Rattus norvegicus | 3 | organism-based format | Scientific Literature |