# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4824527 | Binding | Inhibition of Keap1-Nrf2 (unknown origin) interaction measured after 60 mins by Fluorescence polarization assay | Homo sapiens | 24 | ALA4823275 | protein format | Scientific Literature | |
2. | ALA4824528 | ADME | Cytotoxicity against mouse peritoneal macrophages assessed as reduction in cell viability measured after 24 hrs by CCK8 assay | Mus musculus | 24 | ALA4823275 | cell-based format | Scientific Literature | |
3. | ALA4824529 | Functional | Antiinflammatory activity against mouse peritoneal macrophages assessed as inhibition of LPS-stimulated ROS production at 10 uM incubated for 6 hrs by DCFH-DA staining based fluorescence analysis | Mus musculus | 5 | ALA4823275 | cell-based format | Scientific Literature | |
4. | ALA4824530 | Functional | Antiinflammatory activity against mouse peritoneal macrophages assessed as inhibition of LPS-induced NO production at 10 uM measured after 24 hrs by Griess assay | Mus musculus | 5 | ALA4823275 | cell-based format | Scientific Literature | |
5. | ALA4824531 | Functional | Antiinflammatory activity against mouse peritoneal macrophages assessed as reduction in LPS-induced TNFalpha production at 10 uM measured after 24 hrs by ELISA | Mus musculus | 5 | ALA4823275 | cell-based format | Scientific Literature | |
6. | ALA4824532 | Binding | Inhibition of Keap1-Nrf2 interaction in mouse peritoneal macrophages assessed as increase in nuclear translocation of Nrf2 by measuring increase in nuclear Nrf2 level at 10 uM measured after 3 to 6 hrs by Western blot analysis | Mus musculus | 3 | ALA4823275 | subcellular format | Scientific Literature | |
7. | ALA4824533 | Binding | Inhibition of Keap1-Nrf2 interaction in mouse peritoneal macrophages assessed as increase in nuclear translocation of Nrf2 by measuring decrease in cytosolic Nrf2 level at 10 uM measured after 3 to 6 hrs by Western blot analysis | Mus musculus | 3 | ALA4823275 | subcellular format | Scientific Literature | |
8. | ALA4824534 | Binding | Inhibition of Keap1-Nrf2 interaction in mouse peritoneal macrophages assessed as upregulation of HO-1 protein expression at 10 uM by Western blot analysis | Mus musculus | 3 | ALA4823275 | tissue-based format | Scientific Literature | |
9. | ALA4824535 | Binding | Inhibition of Keap1-Nrf2 interaction in mouse peritoneal macrophages assessed as upregulation of NQO1 protein expression at 10 uM by Western blot analysis | Mus musculus | 3 | ALA4823275 | tissue-based format | Scientific Literature | |
10. | ALA5168791 | Binding | Binding affinity to recombinant N-terminal His6-tagged human KEAP1 Kelch domain (322 to 609 residues) expressed in Escherichia coli BL21 (DE3) by fluorescence anisotropy assay | Homo sapiens | 1 | ALA5154943 | assay format | Scientific Literature | |
11. | ALA5168792 | Binding | Inhibition of recombinant N-terminal His6-tagged human Keap1-Nrf2 (322 to 609 residues) (unknown origin) protein-protein interaction expressed in Escherichia coli (DE3) incubated for 60 mins by fluorescence anisotropy assay | Homo sapiens | 21 | ALA5154943 | assay format | Scientific Literature | |
12. | ALA5168793 | Functional | Cytotoxicity against mouse peritoneal macrophages assessed as reduction in cell viability measured after 24 hrs by Cell Titer-Blue assay | Mus musculus | 21 | ALA5154943 | cell-based format | Scientific Literature | |
13. | ALA5168794 | Functional | Antiinflammatory activity against LPS-induced mouse peritoneal macrophage assessed as suppression of ROS level at 10 uM pretreated with LPS followed by compound addition and measured after 6 hrs by DCFH-DA staining based fluorescence analysis | Mus musculus | 4 | ALA5154943 | cell-based format | Scientific Literature | |
14. | ALA5168795 | Functional | Antiinflammatory activity against LPS-induced mouse peritoneal macrophage assessed as suppression of NO level pretreated with LPS followed by compound addition and measured after 24 hrs by Griess reagent based assay | Mus musculus | 2 | ALA5154943 | cell-based format | Scientific Literature | |
15. | ALA5168796 | Functional | Antiinflammatory activity against LPS-induced mouse peritoneal macrophage assessed as inhibition of TNF alpha level after 4 hrs by ELISA method | Mus musculus | 2 | ALA5154943 | cell-based format | Scientific Literature | |
16. | ALA5168797 | Physicochemical | Aqueous solubility of the compound in PBS at pH 2 after 24 hrs by HPLC-UV analysis | 10 | ALA5154943 | small-molecule physicochemical format | Scientific Literature | ||
17. | ALA5168798 | Physicochemical | Aqueous solubility of the compound in PBS at pH 7 after 24 hrs by HPLC-UV analysis | 10 | ALA5154943 | small-molecule physicochemical format | Scientific Literature | ||
18. | ALA5168799 | Physicochemical | Aqueous solubility of the compound in PBS at pH 7.4 after 24 hrs by HPLC-UV analysis | 10 | ALA5154943 | small-molecule physicochemical format | Scientific Literature | ||
19. | ALA5168823 | Functional | Antiinflammatory activity against LPS-induced mouse peritoneal macrophage assessed as suppression of NO level at 10 uM pretreated with LPS followed by compound addition and measured after 24 hrs by Griess reagent based assay | Mus musculus | 4 | ALA5154943 | cell-based format | Scientific Literature | |
20. | ALA5168824 | Functional | Antiinflammatory activity against LPS-induced mouse peritoneal macrophage assessed as inhibition of TNF alpha level at 10 uM after 4 hrs by ELISA method | Mus musculus | 4 | ALA5154943 | cell-based format | Scientific Literature |