#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA4825443	B	Modulator activity at NF-kappaB in C57BL/6 mouse BMDC cells assessed as increase in cell surface CD40 expression at 200 nM measured after 8 hrs by FACS analysis	Mus musculus	7	ALA4823321	cell-based format	Scientific Literature
2.	ALA4825444	B	Agonist activity at TLR7/8 in mouse RAW-blue cells assessed as immune activation by measuring induction of NFkappaB activation at 500 nM measured after 24 hrs by quanti-blue SEAP reporter gene-based assay	Mus musculus	7	ALA4823321	cell-based format	Scientific Literature
3.	ALA4825445	A	Toxicity in C57BL/6 mouse BMDC cells assessed as induction of inflammation by measuring increase in IL-6 secretion at 200 nM measured after 8 hrs by ELISA	Mus musculus	7	ALA4823321	cell-based format	Scientific Literature
4.	ALA4825446	F	Antioxidant activity in mouse RAW264.7 cells assessed as reduction in intracellular ROS level measured after 16 hrs by CM-H2DCFDA based flow cytometry analysis relative to TLR7/8 SMIP	Mus musculus	6	ALA4823321	cell-based format	Scientific Literature
5.	ALA4825447	F	Inhibition of NO production in mouse RAW264.7 cells at 500 nM incubated for 16 hrs by Griess reagent based analysis	Mus musculus	6	ALA4823321	cell-based format	Scientific Literature
6.	ALA4825448	F	Inhibition of COX-2 expression in mouse RAW264.7 cells at 500 nM incubated for 16 hrs by western blot analysis	Mus musculus	6	ALA4823321	cell-based format	Scientific Literature
7.	ALA4825449	F	Inhibition of iNOS expression in mouse RAW264.7 cells at 500 nM incubated for 16 hrs by western blot analysis	Mus musculus	6	ALA4823321	cell-based format	Scientific Literature
8.	ALA4825450	T	Systemic toxicity in C57BL/6 mouse assessed as increase in serum TNF-alpha level administered intramuscularly in presence of ovalbumin measured after 1 hr	Mus musculus	3	ALA4823321	assay format	Scientific Literature
9.	ALA4825451	F	Immuno-adjuvant activity in C57BL/6 mouse assessed as increase in levels of anti-OVA Ig (A+G+M) administered intramuscularly on day 1 and as booster dose on day 14 in presence of ovalbumin and measured on day 28 by ELISA	Mus musculus	2	ALA4823321	organism-based format	Scientific Literature
10.	ALA4825452	F	Induction of T-cell immunity in C57BL/6 mouse splenocytes assessed as increase in CD3+CD8+ tetramer administered intramuscularly on day 1 and as booster dose on day 14 in presence of ovalbumin and SINFEKL MHC specific tetramer measured on day 28 by flow cytometric analysis	Mus musculus	3	ALA4823321	cell-based format	Scientific Literature
11.	ALA4825453	F	Induction of innate immunity in C57BL/6 mouse naive splenocyte assessed as increase in cell proliferation at 3 uM measured after 48 hrs by MTT assay	Mus musculus	7	ALA4823321	tissue-based format	Scientific Literature
12.	ALA4825454	T	Systemic toxicity in CT26 cells xenografted BALB/c mouse tumor model assessed as increase in serum TNF-alpha level at 20 nmol, IT and measured after 2 hrs	Mus musculus	8	ALA4823321	cell-based format	Scientific Literature
13.	ALA4825455	T	Systemic toxicity in CT26 cells xenografted BALB/c mouse tumor model assessed as increase in serum IL-6 level at 20 nmol, IT and measured after 2 hrs	Mus musculus	9	ALA4823321	cell-based format	Scientific Literature
14.	ALA4825456	T	Systemic toxicity in CT26 cells xenografted BALB/c mouse tumor model assessed as reduction in white blood count in peripheral blood at 20 nmol, IT and measured after 24 hrs by hematological analysis	Mus musculus	8	ALA4823321	cell-based format	Scientific Literature
15.	ALA4825458	T	Systemic toxicity in C57BL/6 mouse assessed as increase in serum IL-6 level administered intramuscularly in presence of ovalbumin measured after 1 hr	Mus musculus	3	ALA4823321	assay format	Scientific Literature
16.	ALA4825459	F	Immuno-adjuvant activity in C57BL/6 mouse assessed as increase in levels of anti-OVA IgG administered intramuscularly on day 1 and as booster dose on day 14 in presence of ovalbumin and measured on day 28 by ELISA	Mus musculus	2	ALA4823321	organism-based format	Scientific Literature
17.	ALA4825461	T	Systemic toxicity in CT26 cells xenografted BALB/c mouse tumor model assessed as reduction in lymphocyte count in peripheral blood at 20 nmol, IT and measured after 24 hrs by hematological analysis	Mus musculus	9	ALA4823321	cell-based format	Scientific Literature