# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4834652 | Binding | Inhibition of recombinant full length N-terminal His6-tagged LTA4H (unknown origin) expressed in Escherichia coli BL21 DE3 cells at enzyme concentration 9 nM using Arg-AMC as substrate preincubated for 15 mins followed by substrate addition and measured every 10 mins for 300 mins by fluorescence based assay | Homo sapiens | 27 | ALA4834397 | cell-based format | Scientific Literature | |
2. | ALA4834661 | Binding | Inhibition of LTA4H in human whole blood assessed as reduction of calcium ionophore A23187-stimulated LTB4 generation preincubated for 15 mins followed by ionophore stimulation and measured after 15 hrs by EIA assay | Homo sapiens | 25 | ALA4834397 | tissue-based format | Scientific Literature | |
3. | ALA4834662 | ADME | Half life in rat liver microsomes in presence of NADPH | Rattus norvegicus | 12 | ALA4834397 | microsome format | Scientific Literature | |
4. | ALA4834663 | Binding | Displacement of [3H]dofetilide from recombinant human ERG stably expressed in HEK293 cell membranes measured after 90 mins by microbeta liquid scintillation counting method | Homo sapiens | 13 | ALA4834397 | cell membrane format | Scientific Literature | |
5. | ALA4834664 | ADME | Reversible inhibition of CYP2C9 in human liver microsomes using midazolam as substrate incubated for 10 mins in presence of NADPH by LC-MS analysis | Homo sapiens | 12 | ALA4834397 | microsome format | Scientific Literature | |
6. | ALA4834665 | ADME | Reversible inhibition of CYP2D6 in human liver microsomes using midazolam as substrate incubated for 10 mins in presence of NADPH by LC-MS analysis | Homo sapiens | 12 | ALA4834397 | microsome format | Scientific Literature | |
7. | ALA4834666 | ADME | Reversible inhibition of CYP3A5 in human liver microsomes using midazolam as substrate incubated for 10 mins in presence of NADPH by LC-MS analysis | Homo sapiens | 12 | ALA4834397 | microsome format | Scientific Literature | |
8. | ALA4834667 | Binding | Displacement of [3H]WIN35428 from recombinant human dopamine transporter by radioligand binding assay | Homo sapiens | 15 | ALA4834397 | single protein format | Scientific Literature | |
9. | ALA4834668 | Physicochemical | Solubility of compound in aqueous buffer at pH 6.8 by HPLC analysis | 15 | ALA4834397 | small-molecule physicochemical format | Scientific Literature | ||
10. | ALA4834669 | Functional | Ex-vivo inhibition of LTA4H in blood isolated from C57/BL6 mouse assessed as reduction of calcium ionophore A23187-stimulated LTB4 generation at 10 mg/kg, po measured after 14 hrs by EIA assay | Mus musculus | 15 | ALA4834397 | organism-based format | Scientific Literature |