#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA4827795	Binding	Displacement of fluorophore-labeled RIOK1 from human PRMT5/WDR77 hetero octameric complex expressed in Sf9 cells by competition fluorescence polarization (FP) assay	Homo sapiens	33	ALA4825730	cell-based format	Scientific Literature
2.	ALA4827796	Physicochemical	Solubility in PBS buffer		33	ALA4825730	small-molecule physicochemical format	Scientific Literature
3.	ALA4827797	ADME	Stability in human plasma assessed as compound remaining relative to control	Homo sapiens	33	ALA4825730	cell-free format	Scientific Literature
4.	ALA4827798	ADME	Stability in mouse plasma assessed as compound remaining relative to control	Mus musculus	33	ALA4825730	cell-free format	Scientific Literature
5.	ALA4827799	Physicochemical	Glutathione reactivity assessed as stability by measuring glutathione half life at 10 uM measured up to 1440 mins by LC-MS/MS analysis		12	ALA4825730	small-molecule physicochemical format	Scientific Literature
6.	ALA4827806	Binding	Covalent inhibition of human PRMT5 assessed maximum potential inactivation rate by LC-MS analysis	Homo sapiens	8	ALA4825730	single protein format	Scientific Literature
7.	ALA4827807	Binding	Covalent inhibition of human PRMT5 assessed as initial binding constant by LC-MS analysis	Homo sapiens	8	ALA4825730	single protein format	Scientific Literature
8.	ALA4827808	Binding	Covalent inhibition of human PRMT5 assessed as Kinact/Ki ratio by LC-MS analysis	Homo sapiens	8	ALA4825730	single protein format	Scientific Literature
9.	ALA4827809	Binding	Activity at human wild type PRMT5/HA-tagged WDR77 hetero octameric complex expressed in HEK293 (Expi293) cells assessed as PRMT5 adduct formation incubated for 6 hrs by LC-MS analysis	Homo sapiens	1	ALA4825730	cell-based format	Scientific Literature
10.	ALA4827810	Binding	Disruption of human N-terminal SmBiT peptide-tagged PRMT5/ N-terminal LgBiT tagged RIOK1 complex expressed in permeabilized HEK293T cells by NanoBiT assay	Homo sapiens	2	ALA4825730	cell-based format	Scientific Literature
11.	ALA4827811	Binding	Disruption of human N-terminal SmBiT peptide-tagged PRMT5/N-terminal LgBiT tagged RIOK1 complex expressed in intact HEK293T cells measured after 40 mins by NanoBiT assay	Homo sapiens	2	ALA4825730	cell-based format	Scientific Literature
12.	ALA4827812	Binding	Inhibition of PRMT5 methyltransferase activity in human MTAP -/- HCT-116 cells assessed as total symmetric arginine dimethylation levels by Western blot analysis	Homo sapiens	1	ALA4825730	tissue-based format	Scientific Literature
13.	ALA5218139	Binding	Inhibition of full length human ICLN/PRMT5 (unknown origin) protein-protein interaction by fluorescence polarization assay	Homo sapiens	2	ALA5214998	assay format	Scientific Literature