#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA4817609	B	Inhibition of recombinant human His-tagged DCN1 (58 to 259 residues) expressed in Escherichia coli BL21 (DE3) assessed as reduction in DCN1/UBC12 interaction incubated for 30 mins by fluorescence polarization assay	Homo sapiens	40	ALA4814015	assay format	Scientific Literature
2.	ALA4817610	B	Inhibition of recombinant human His-tagged DCN1 (58 to 259 residues) expressed in Escherichia coli BL21 (DE3) using MIKLFSLKQQKKEEESAGGTK-biotin as substrate incubated for 30 mins by HTRF assay	Homo sapiens	40	ALA4814015	assay format	Scientific Literature
3.	ALA4817611	P	Aqueous solubility of compound in PBS at pH 7.4 incubated for 24 hrs by spectroscopic analysis		1	ALA4814015	small-molecule physicochemical format	Scientific Literature
4.	ALA4817612	B	Binding affinity to recombinant human His-tagged DCN1 (58 to 259 residues) expressed in Escherichia coli BL21 (DE3) assessed as increase in thermal stability by thermal shift assay	Homo sapiens	1	ALA4814015	assay format	Scientific Literature
5.	ALA4817613	B	Inhibition of recombinant human GST-tagged DCN2 (62 to 259 residues) expressed in Escherichia coli BL21 (DE3) using MIKLFSLKQQKKEEESAGGTK-biotin as substrate incubated for 30 mins by HTRF assay	Homo sapiens	1	ALA4814015	assay format	Scientific Literature
6.	ALA4817614	B	Inhibition of recombinant human GST-tagged DCN3 (86 to 304 residues) expressed in Escherichia coli BL21 (DE3) using MIKLFSLKQQKKEEESAGGTK-biotin as substrate incubated for 30 mins by HTRF assay	Homo sapiens	1	ALA4814015	assay format	Scientific Literature
7.	ALA4817615	B	Inhibition of recombinant human GST-tagged DCN4 (102 to 292 residues) expressed in Escherichia coli BL21 (DE3) using MIKLFSLKQQKKEEESAGGTK-biotin as substrate incubated for 30 mins by HTRF assay	Homo sapiens	1	ALA4814015	assay format	Scientific Literature
8.	ALA4817616	B	Inhibition of recombinant human GST-tagged DCN5 (47 to 237 residues) expressed in Escherichia coli BL21 (DE3) using MIKLFSLKQQKKEEESAGGTK-biotin as substrate incubated for 30 mins by HTRF assay	Homo sapiens	1	ALA4814015	assay format	Scientific Literature
9.	ALA4817618	B	Inhibition of NAE1 (unknown origin)-mediated Nedd8-Ubcl2 adduct formation at 100 uM incubated for 10 mins followed by Mg-ATP addition and measured after 60 mins by Western blot analysis	Homo sapiens	1	ALA4814015	single protein format	Scientific Literature
10.	ALA4817619	B	Binding affinity to DCN1 in human NCI-H2170 cells assessed as increase in thermal stability measured between 43 and 64 deg C at 10 uM incubated for 3 to 5 hrs by cellular thermal shift assay	Homo sapiens	2	ALA4814015	cell-based format	Scientific Literature
11.	ALA4817620	B	Binding affinity to DCN1 in human KYSE-70 cell line assessed as increase in thermal stability at 10 uM incubated for 3 to 5 hrs by cellular thermal shift assay	Homo sapiens	2	ALA4814015	tissue-based format	Scientific Literature
12.	ALA4817621	B	Binding affinity to DCN1 in human NCI-H2170 cells assessed as increase in thermal stability measured 52 deg C at 10 uM incubated for 3 to 5 hrs by cellular thermal shift assay	Homo sapiens	1	ALA4814015	cell-based format	Scientific Literature
13.	ALA4817622	B	Inhibition of DCN-1 in human KYSE-70 cell line assessed as increase in NRF2 expression at 2.5 to 20 uM incubated for 24 hrs by Western blot analysis	Homo sapiens	1	ALA4814015	tissue-based format	Scientific Literature
14.	ALA4817623	B	Inhibition of DCN-1 in human KYSE-70 cell line assessed as increase in HO-1 expression at 2.5 to 20 uM incubated for 24 hrs by Western blot analysis	Homo sapiens	1	ALA4814015	tissue-based format	Scientific Literature
15.	ALA4817624	B	Inhibition of DCN-1 in human NCI-H2170 cells assessed as reduction in CUL-3 neddylation at 2.5 to 20 uM incubated for 24 hrs by Western blot analysis	Homo sapiens	1	ALA4814015	cell-based format	Scientific Literature
16.	ALA4817625	B	Inhibition of DCN-1 in human NCI-H2170 cells assessed as increase in NRF2 expression at 2.5 to 20 uM incubated for 24 hrs by Western blot analysis	Homo sapiens	1	ALA4814015	cell-based format	Scientific Literature
17.	ALA4817626	B	Inhibition of DCN-1 in human NCI-H2170 cells assessed as increase in HO-1 expression at 2.5 to 20 uM incubated for 24 hrs by Western blot analysis	Homo sapiens	1	ALA4814015	cell-based format	Scientific Literature
18.	ALA4817629	F	Cytotoxicity against human SMMC-7721 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	Homo sapiens	3	ALA4814015	cell-based format	Scientific Literature
19.	ALA4817630	F	Cytotoxicity against human EC9706 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	Homo sapiens	3	ALA4814015	cell-based format	Scientific Literature
20.	ALA4817631	F	Cytotoxicity against human TE-1 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	Homo sapiens	3	ALA4814015	cell-based format	Scientific Literature