# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4845096 | Binding | Inhibition of full length human CDK2 (1 to 298 end residues)/N-terminal GST-tagged CyclinA2 (1 to 432 residues) expressed in baculovirus expression system at 100 nM preincubated for 10 mins followed by substrate and ATP addition by mobility shift assay relative to control | Homo sapiens | 31 | ALA4842385 | assay format | Scientific Literature | |
2. | ALA4845097 | Binding | Inhibition of full length human CDK4 (1 to 303 residues)/N-terminal GST-fusion tagged CyclinD3 (1 to 292 residues) expressed in baculovirus expression system at 100 nM preincubated for 10 mins followed by substrate and ATP addition by mobility shift assay relative to control | Homo sapiens | 31 | ALA4842385 | assay format | Scientific Literature | |
3. | ALA4845098 | Binding | Inhibition of full length human CDK6 (1 to 326 end residues)/N-terminal GST-fusion tagged CyclinD3 (1 to 292 residues) expressed in baculovirus expression system at 100 nM preincubated for 10 mins followed by substrate and ATP addition by mobility shift assay relative to control | Homo sapiens | 31 | ALA4842385 | assay format | Scientific Literature | |
4. | ALA4845099 | Binding | Inhibition of HDAC in human HeLa nuclear extract at 1000 nM using Boc-Lys(acetyl)-AMC as substrate measured after 1 hr by fluorescence microplate reader assay relative to control | Homo sapiens | 31 | ALA4842385 | subcellular format | Scientific Literature | |
5. | ALA4845100 | Binding | Inhibition of HDAC1 (unknown origin) measured after 30 mins by fluorescence microplate reader assay | Homo sapiens | 5 | ALA4842385 | single protein format | Scientific Literature | |
6. | ALA4845101 | Binding | Inhibition of HDAC2 (unknown origin) measured after 30 mins by fluorescence microplate reader assay | Homo sapiens | 5 | ALA4842385 | single protein format | Scientific Literature | |
7. | ALA4845102 | Binding | Inhibition of C-terminal His-tagged recombinant human HDAC3 (1 to 428 residues)/N-terminal GST-tagged recombinant human NCoR2 (395 to 489 residues) co-expressed in baculovirus infected Sf9 cells measured after 30 mins by fluorescence microplate reader assay | Homo sapiens | 5 | ALA4842385 | cell-based format | Scientific Literature | |
8. | ALA4845103 | Binding | Inhibition of HDAC4 (unknown origin) measured after 30 mins by fluorescence microplate reader assay | Homo sapiens | 5 | ALA4842385 | single protein format | Scientific Literature | |
9. | ALA4845104 | Binding | Inhibition of HDAC5 (unknown origin) measured after 30 mins by fluorescence microplate reader assay | Homo sapiens | 5 | ALA4842385 | single protein format | Scientific Literature | |
10. | ALA4845105 | Binding | Inhibition of recombiant human HDAC6 using fluorogenic HDAC substrate 3 measured after 30 mins by fluorescence microplate reader assay | Homo sapiens | 5 | ALA4842385 | single protein format | Scientific Literature | |
11. | ALA4845106 | Binding | Inhibition of HDAC7 (unknown origin) measured after 30 mins by fluorescence microplate reader assay | Homo sapiens | 5 | ALA4842385 | single protein format | Scientific Literature | |
12. | ALA4845107 | Binding | Inhibition of HDAC8 (unknown origin) measured after 30 mins by fluorescence microplate reader assay | Homo sapiens | 5 | ALA4842385 | single protein format | Scientific Literature | |
13. | ALA4845108 | Binding | Inhibition of recombinant human HDAC9 measured after 30 mins by fluorescence microplate reader assay | Homo sapiens | 5 | ALA4842385 | single protein format | Scientific Literature | |
14. | ALA4845109 | Binding | Inhibition of recombinant human HDAC11 measured after 30 mins by fluorescence microplate reader assay | Homo sapiens | 5 | ALA4842385 | single protein format | Scientific Literature | |
15. | ALA4845110 | Binding | Inhibition of recombinant human SIRT2 measured after 30 mins by fluorescence microplate reader assay | Homo sapiens | 5 | ALA4842385 | single protein format | Scientific Literature | |
16. | ALA4845111 | Binding | Inhibition of recombinant human SIRT5 measured after 30 mins by fluorescence microplate reader assay | Homo sapiens | 5 | ALA4842385 | single protein format | Scientific Literature | |
17. | ALA4845112 | Binding | Inhibition of full length human CDK2 (1 to 298 end residues)/N-terminal GST-tagged CyclinA2 (1 to 432 residues) expressed in baculovirus expression system preincubated for 10 mins followed by substrate and ATP addition by mobility shift assay | Homo sapiens | 5 | ALA4842385 | assay format | Scientific Literature | |
18. | ALA4845113 | Binding | Inhibition of full length human CDK4 (1 to 303 residues)/N-terminal GST-fusion tagged CyclinD3 (1 to 292 residues) expressed in baculovirus expression system preincubated for 10 mins followed by substrate and ATP addition by mobility shift assay | Homo sapiens | 5 | ALA4842385 | assay format | Scientific Literature | |
19. | ALA4845114 | Binding | Inhibition of full length human CDK6 (1 to 326 end residues)/N-terminal GST-fusion tagged CyclinD3 (1 to 292 residues) expressed in baculovirus expression system preincubated for 10 mins followed by substrate and ATP addition by mobility shift assay | Homo sapiens | 5 | ALA4842385 | assay format | Scientific Literature | |
20. | ALA4845115 | Functional | Antiproliferative activity against human HepG2 cells assessed as cell growth inhibition measured after 48 hrs by CCK8 assay | Homo sapiens | 5 | ALA4842385 | cell-based format | Scientific Literature |