#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA4831306	Binding	Inhibition of CDC7 in human COLO 205 cells assessed as reduction in phosphorylation of MCM2 at 1 uM preincubated for 48 hrs followed by compound washout measured after 24 hrs by Western blot analysis	Homo sapiens	1	ALA4828769	cell-based format	Scientific Literature
2.	ALA4831307	Binding	Reversible inhibition of human CDC7 assessed as off-rate preincubated for 50 mins followed by substrate addition in presence of ATP by rapid dilution assay	Homo sapiens	1	ALA4828769	single protein format	Scientific Literature
3.	ALA4831308	Binding	Inhibition of human CDC7/ASK using FITC-labelled MCM2 substrate in presence of 5 uM of ATP	Homo sapiens	24	ALA4828769	assay format	Scientific Literature
4.	ALA4831309	Binding	Inhibition of human CDC7/ASK using FITC-labelled MCM2 substrate in presence of 1 mM of ATP	Homo sapiens	24	ALA4828769	assay format	Scientific Literature
5.	ALA4831310	Binding	Inhibition of human CDC7/ASK using FITC-labelled MCM2 substrate preincubated for 30 mins followed by substrate addition and measured after 5 hrs in presence of 1 mM of ATP	Homo sapiens	24	ALA4828769	assay format	Scientific Literature
6.	ALA4831311	Binding	Inhibition of CDC7 in human COLO205 cells assessed as reduction in MCM2 phosphorylation at S53 residue at 0.1 uM incubated for 48 hrs by Western blot analysis relative to control	Homo sapiens	8	ALA4828769	cell-based format	Scientific Literature
7.	ALA4831312	Functional	Induction of cell cycle arrest in human COLO205 cells assessed as accumulation of cells at sub-G1 phase at 1 uM incubated for 48 hrs by propidium iodide staining based flow cytometry analysis	Homo sapiens	8	ALA4828769	cell-based format	Scientific Literature
8.	ALA4831313	ADME	Metabolic stability in human liver microsomes assessed as parent compound remaining after 30 mins	Homo sapiens	2	ALA4828769	microsome format	Scientific Literature
9.	ALA4831314	ADME	Metabolic stability in rat liver microsomes assessed as parent compound remaining after 30 mins	Rattus norvegicus	1	ALA4828769	microsome format	Scientific Literature
10.	ALA4831315	ADME	Metabolic stability in dog liver microsomes assessed as parent compound remaining after 30 mins	Canis lupus familiaris	1	ALA4828769	microsome format	Scientific Literature
11.	ALA4831316	ADME	Metabolic stability in mouse liver microsomes assessed as parent compound remaining after 30 mins	Mus musculus	2	ALA4828769	microsome format	Scientific Literature
12.	ALA4831317	ADME	Apparent permeability across apical to basolateral side in human Caco-2 cells	Homo sapiens	1	ALA4828769	cell-based format	Scientific Literature
13.	ALA4831318	ADME	Protein binding in human plasma assessed as unbound fraction	Homo sapiens	1	ALA4828769	cell-free format	Scientific Literature
14.	ALA4831319	Binding	Inhibition of PIM1 (unknown origin) at 0.1 uM in presence of 1 mM ATP relative to control	Homo sapiens	1	ALA4828769	single protein format	Scientific Literature
15.	ALA4831320	Binding	Inhibition of GSK3alpha (unknown origin) at 0.1 uM in presence of 1 mM ATP relative to control	Homo sapiens	1	ALA4828769	single protein format	Scientific Literature
16.	ALA4831321	Binding	Inhibition of CLK1 (unknown origin) at 0.1 uM in presence of 1 mM ATP relative to control	Homo sapiens	1	ALA4828769	single protein format	Scientific Literature
17.	ALA4831322	Binding	Inhibition of CLK2 (unknown origin) at 0.1 uM in presence of 1 mM ATP relative to control	Homo sapiens	1	ALA4828769	single protein format	Scientific Literature
18.	ALA4831323	Binding	Selectivity index, ratio of IC50 for inhibition of human CDC7 in presence of 1 mM ATP without preincubation to IC50 for inhibition of human CDC7 using FITC-labelled MCM2 substrate preincubated for 30 mins followed by substrate addition and measured after 5 hrs in presence of 1 mM ATP	Homo sapiens	1	ALA4828769	assay format	Scientific Literature
19.	ALA4831324	Binding	Inhibition of PIM1 (unknown origin) in presence of 1 mM ATP without preincubation	Homo sapiens	1	ALA4828769	single protein format	Scientific Literature
20.	ALA4831325	Binding	Inhibition of PIM1 (unknown origin) in presence of 1 mM ATP with preincubation	Homo sapiens	1	ALA4828769	single protein format	Scientific Literature