#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA4831635	B	Binding affinity to recombinant His-tagged ERalpha LBD (307 to 554 residue) (unknown origin) expressed in Escherichia coli preincubated for 15 mins followed by ligand addition and measured after 1 hr by fluorescent polarization assay	Homo sapiens	23		assay format	Scientific Literature
2.	ALA4831636	B	Binding affinity to ERalpha Y537S mutant (unknown origin) expressed in Escherichia coli preincubated for 15 mins followed by ligand addition and measured after 1 hr by fluorescent polarization assay	Homo sapiens	16		assay format	Scientific Literature
3.	ALA4831637	B	Binding affinity to ERalpha S463P mutant (unknown origin) expressed in Escherichia coli preincubated for 15 mins followed by ligand addition and measured after 1 hr by fluorescent polarization assay	Homo sapiens	16		assay format	Scientific Literature
4.	ALA4831638	B	Binding affinity to ERalpha D538G mutant (unknown origin) expressed in Escherichia coli preincubated for 15 mins followed by ligand addition and measured after 1 hr by fluorescent polarization assay	Homo sapiens	16		assay format	Scientific Literature
5.	ALA4831639	F	Antiproliferative activity against human MCF7 cells assessed as growth inhibition preincubated with estradiol for 30 mins followed by compound addition and measured after 72 hrs by CellTiter-Glo assay	Homo sapiens	23		cell-based format	Scientific Literature
6.	ALA4831640	F	Antiproliferative activity against human MCF7 cells assessed as growth inhibition preincubated with estradiol for 30 mins followed by compound addition and measured after 7 days by CellTiter-Glo assay	Homo sapiens	23		cell-based format	Scientific Literature
7.	ALA4831642	B	Induction of ERalpha degradation in human MCF7 cells assessed as decrease in ERalpha protein expression incubated for 24 hrs by Western blot analysis	Homo sapiens	3		cell-based format	Scientific Literature
8.	ALA4831643	B	Induction of ERalpha degradation in human MCF7 cells assessed as decrease in ERalpha protein expression incubated for 48 hrs by Western blot analysis	Homo sapiens	9		cell-based format	Scientific Literature
9.	ALA4831646	B	Antagonist activity at ERalpha in human MCF7 cells assessed as reduction in PGR gene expression incubated for 24 hrs by qPCR analysis	Homo sapiens	2		cell-based format	Scientific Literature
10.	ALA4831647	F	Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability preincubated with estradiol for 30 mins followed by compound addition and measured after 7 days by CellTiter-Glo assay	Homo sapiens	2		cell-based format	Scientific Literature
11.	ALA4831649	B	Induction of ERalpha degradation in human MCF7 cells assessed as reduction in ERalpha expression at 1 uM after 2 hrs by Western blotting analysis	Homo sapiens	1		cell-based format	Scientific Literature
12.	ALA4831650	B	Induction of ERalpha degradation in human MCF7 cells assessed as decrease in ERalpha protein expression preincubated for 2 hrs followed by compound washout and measured after 22 hrs by Western blotting analysis	Homo sapiens	2		cell-based format	Scientific Literature
13.	ALA4831651	B	Induction of ERalpha degradation in human MCF7 cells assessed as decrease in ERalpha protein expression incubated for 48 hrs in presence of VHL298 by Western blotting analysis	Homo sapiens	2		cell-based format	Scientific Literature
14.	ALA4831652	B	Selectivity index, ratio of DC50 for induction of ERalpha degradation in human MCF7 cells in presence of VHL298 to DC50 for induction of ERalpha degradation in human MCF7 cells	Homo sapiens	2		cell-based format	Scientific Literature
15.	ALA4831653	B	Induction of ERalpha degradation in human T47D cells assessed as decrease in ERalpha protein expression incubated for 24 hrs by Western blotting analysis	Homo sapiens	2		cell-based format	Scientific Literature
16.	ALA4831654	F	Antiproliferative activity against human MCF7 cells assessed as growth inhibition incubated for 7 days by CellTiter-Glo assay	Homo sapiens	2		cell-based format	Scientific Literature
17.	ALA4831655	F	Antiproliferative activity against human T47D cells assessed as growth inhibition incubated for 7 days by CellTiter-Glo assay	Homo sapiens	2		cell-based format	Scientific Literature
18.	ALA4831656	F	Antiproliferative activity against human ZR-75-1 cells assessed as growth inhibition incubated for 7 days by CellTiter-Glo assay	Homo sapiens	2		cell-based format	Scientific Literature
19.	ALA4831657	F	Antiproliferative activity against human MDA-MB-231 cells assessed as growth inhibition incubated for 7 days by CellTiter-Glo assay	Homo sapiens	2		cell-based format	Scientific Literature
20.	ALA4831658	A	Cytotoxicity against human MCF-10A cells assessed as growth inhibition incubated for 7 days by CellTiter-Glo assay	Homo sapiens	2		cell-based format	Scientific Literature