# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4812574 | Binding | Inhibition of BACE-1 (unknown origin) at 2 uM using Mca-Ser-Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Lys-Dnp-OH as substrate preincubated for 30 mins followed by substrate addition and measured after 1 hr by FRET assay relative to control | Homo sapiens | 15 | ALA4811256 | single protein format | Scientific Literature | |
2. | ALA4812575 | Binding | Inhibition of BACE-1 (unknown origin) using Mca-Ser-Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Lys-Dnp-OH as substrate preincubated for 30 mins followed by substrate addition and measured after 1 hr by FRET assay | Homo sapiens | 15 | ALA4811256 | single protein format | Scientific Literature | |
3. | ALA4812576 | Functional | Glutathione peroxidase-like activity in pH 7.5 potassium phosphate buffer assessed as catalytic reduction of H2O2 by measuring initial velocity at 25 degreeC using GSH in presence of NADPH by UV absorbance analysis (Rvb = 30.41+/- 2.06 microM/min) | 15 | ALA4811256 | assay format | Scientific Literature | ||
4. | ALA4812577 | ADME | Cytotoxicity against rat PC-12 cells at 25 uM incubated for 24 hrs by MTT assay | Rattus norvegicus | 6 | ALA4811256 | cell-based format | Scientific Literature | |
5. | ALA4812578 | Functional | Neuroprotective activity against H2O2-induced cell damage in rat PC-12 cells assessed as decrease in cell death at 10 uM pretreated for 12 hrs followed by H2O2 challenge by MTT assay | Rattus norvegicus | 8 | ALA4811256 | cell-based format | Scientific Literature |